<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Makhele Thapelo Simon</style></author><author><style face="normal" font="default" size="100%">Makhoahle Pakiso Moses</style></author><author><style face="normal" font="default" size="100%">Mashele Sitheni Samson</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Anti-Neoplastic and Cytotoxicity Potency Measuring of Five Medicago sativa L. (Alfalfa) Leaf Extracts Towards Melanoma (UACC62), Breast (MCF7), Prostate (PC3), and Colon (HCT116) Cancer Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AICR (American Institute for Cancer Research)</style></keyword><keyword><style  face="normal" font="default" size="100%">Cancer (malignancy</style></keyword><keyword><style  face="normal" font="default" size="100%">Carcinoma</style></keyword><keyword><style  face="normal" font="default" size="100%">CSIR (The Council for Scientific and Industrial Research</style></keyword><keyword><style  face="normal" font="default" size="100%">ECACC (European Collection of Authenticated Cell Cultures</style></keyword><keyword><style  face="normal" font="default" size="100%">International Agency for Research on Cancer (IARC)</style></keyword><keyword><style  face="normal" font="default" size="100%">Leukemia</style></keyword><keyword><style  face="normal" font="default" size="100%">lymphoma)</style></keyword><keyword><style  face="normal" font="default" size="100%">Medicago sativa L. (Alfalfa)</style></keyword><keyword><style  face="normal" font="default" size="100%">NCI (National Cancer Institute</style></keyword><keyword><style  face="normal" font="default" size="100%">neoplasm</style></keyword><keyword><style  face="normal" font="default" size="100%">sarcoma</style></keyword><keyword><style  face="normal" font="default" size="100%">South Africa)</style></keyword><keyword><style  face="normal" font="default" size="100%">Sulforhodamine B Assay (SRB)</style></keyword><keyword><style  face="normal" font="default" size="100%">THC (delta-9 tetrahydrocannabinol). Rick Simpson oil (RSO).</style></keyword><keyword><style  face="normal" font="default" size="100%">Tumour</style></keyword><keyword><style  face="normal" font="default" size="100%">U.S Department of Health and Human Services)</style></keyword><keyword><style  face="normal" font="default" size="100%">UK)</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">October 2023</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">768-776</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;The National Cancer Registry of the National Department of Health of South Africa confirms that in females, the highest incidence of cancers diagnosed in the year 2019 was breast (10 172 cases), cervical (6 945 cases) and colorectal (1 952 cases) cancers, while in males the most diagnosed cancers were prostate, lung, and colorectal neoplasms. The risk factors that promote cancer development, and metastasis or spreading includes excessive alcohol and narcotic usage, carcinogenic infections (oncoviruses) and genetical predispositions. The global incidence of cancer is much likely to increase because of the increased exposure to risk factors (processed fast foods, industrial toxins in air and water) that promote oxidative stress, low grade chronic inflammation, diabetes mellitus, hyperglycemia, and insulin resistance. Because the ancient healer or doctor relied on plants and other natural resources for therapeutic compounds, we wanted to measure the anti-cancer actions induced by our Alfac-facah leaf extracts towards four major cancer cells. The growth inhibitory and cytotoxicity activity that was induced by our extracts was measured using the reliable Sulforhodamine B Assay as per the collaborative research program between the CSIR’s Biosciences Pharmacology Group and the NCI. Our Alfac-facah leaf test material was extracted using five solvents: Ethanol, Methanol, Diethyl-ether, Acetone, and Water. Medicinal Plants persist to play a key role in medicine, whereby they not only help with treating or preventing diseases, but they also contribute to the general wellbeing of the patient. For this reason, natural resources remain a pivotal ingredient of novel drug development compounds&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">768</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Makhele Thapelo Simon, Makhoahle Pakiso Moses*, Mashele Sitheni Samson&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;Faculty of Health and Environmental Sciences, Central University of Technology, Free State Province, 20 President Brand Street, Bloemfontein, SOUTH AFRICA.&lt;/p&gt;
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