<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Shahad Mohammed Nasser Alqahtani</style></author><author><style face="normal" font="default" size="100%">Dalya Ramzi Alsuliman</style></author><author><style face="normal" font="default" size="100%">Abdullah Jalal Alasoom</style></author><author><style face="normal" font="default" size="100%">Hussein Ali Burshed</style></author><author><style face="normal" font="default" size="100%">Marwan Mohamed Alshawush</style></author><author><style face="normal" font="default" size="100%">Abdullah Abdulhamid Altaweel</style></author><author><style face="normal" font="default" size="100%">Hany Ezzat Khalil</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cichorioside a biocoumarin modulates lipid and glucose storage on 3T3-L1 cell lines: In vitro and in silico approach</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">3T3-L1cell lines</style></keyword><keyword><style  face="normal" font="default" size="100%">Cichorioside</style></keyword><keyword><style  face="normal" font="default" size="100%">Oil red O staining</style></keyword><keyword><style  face="normal" font="default" size="100%">PPARγ</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">January 2023</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">933-937</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;Natural coumarins are reputed to demonstrate several biological effects to improve adverse health conditions including; obesity. This study was conducted to explore the potential&lt;em&gt; in vitro&lt;/em&gt; lipid and glucose modulatory activity of cichorioside on 3T3-L1 cell lines .&lt;strong&gt; Methods: &lt;/strong&gt;The 3T3-L1 cell lines were cultured and cell viability was assessed. Glucose content in medium of cultured cells was measured. Differentiation of 3T3-L1 cells from pre-adipocytes to adipocytes was evaluated upon addition of cichorioside. Expression of mRNA of the peroxisome proliferator-activated receptor gamma (PPARγ) was estimated. &lt;strong&gt;Results: &lt;/strong&gt;Survival of around 90% of cultured cells was observed at 10μM cichorioside. Cichorioside inhibited glucose uptake from the medium by the 3T3-L1 cell lines. Cichorioside considerably inhibited pre-adipocyte differentiation and the lipid content in intercellular storage. Cichorioside demonstrated an upregulation of the mRNA expression of PPARγ. Moreover, the docking studies supported the results &lt;em&gt;via &lt;/em&gt;the deep interaction of cichorioside with amino acids residue of PPAR-γ. Taken together, these findings are the first report on &lt;em&gt;in vitro&lt;/em&gt; evaluation of cichorioside to modulate the lipid storage and glucose uptake of cultured 3T3-L1 cell lines&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6s</style></issue><work-type><style face="normal" font="default" size="100%">Original Article </style></work-type><section><style face="normal" font="default" size="100%">933</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Shahad Mohammed Nasser Alqahtani*, Dalya Ramzi Alsuliman, Abdullah Jalal Alasoom, Hussein Ali Burshed, Marwan Mohamed Alshawush, Abdullah Abdulhamid Altaweel, Hany Ezzat Khalil*&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa 31982, SAUDI ARABIA.&lt;/p&gt;
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