<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Aditi Gupta</style></author><author><style face="normal" font="default" size="100%">Sunil Kumar</style></author><author><style face="normal" font="default" size="100%">Neeraj Mahindroo</style></author><author><style face="normal" font="default" size="100%">Reena Vohra Saini</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Bioactive Fraction from Datura stramonium Linn. Promotes Human immune Cells Mediated Cytotoxicity towards Lung and Breast Cancer Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anticancer</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytokine</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxic</style></keyword><keyword><style  face="normal" font="default" size="100%">Datura stramonium.</style></keyword><keyword><style  face="normal" font="default" size="100%">Immunomodulation</style></keyword><keyword><style  face="normal" font="default" size="100%">PBMC</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Oct 2016</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">8</style></volume><pages><style face="normal" font="default" size="100%">435-439</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Aim: &lt;/strong&gt;The aim of the present study was to evaluate immune modulatory effect of fractions of &lt;em&gt;D. stramonium&lt;/em&gt; L. leaves on human peripheral blood mononuclear cells (PBMC) followed by assessment of cytotoxic abilities of immunomodulated PBMC toward cancer cells. &lt;strong&gt;Material and methods: &lt;/strong&gt;Bioassay (PBMC proliferation) guided fractionation of methanolic leaf extract of &lt;em&gt;D. stramonium&lt;/em&gt; was performed to get active fraction and LC-MS was performed to identify the phytocompounds present in the bioactive fraction. The immunomodulatory potential of&lt;em&gt; D.&lt;/em&gt; &lt;em&gt;stramonium&lt;/em&gt; active fraction was assessed by i) MTT microcytotoxicity assay using A549 (lung carcinomas) and MCF-7 (breast cancer) cell lines and ii) analyzing the production of IL-2 and IFN-&amp;gamma; by human PBMC in the presence of active fraction. &lt;strong&gt;Results:&lt;/strong&gt; Chromatographic fractionation guided by PBMC proliferation assay of &lt;em&gt;D. stramonium&lt;/em&gt; extract resulted in bioactive fraction (fraction-10) exhibiting significant immunostimulatory activity [EC&lt;sub&gt;50&lt;/sub&gt;=19.1&amp;plusmn;1.5 (&amp;mu;g/ml)] on human blood lymphocytes. Fraction-10 pretreated PBMC displayed enhanced cytotoxicity towards A549 and MCF-7 (59%&amp;plusmn;2.1% and 62%&amp;plusmn;2.3% at 1:20 effector:target ratio respectively). Moreover, fraction-10 also enhanced the secretion of IL-2 (8 fold) and IFN-&amp;gamma; (10 fold) by human PBMC. The preliminary phytochemical analysis of fraction-10 from&lt;em&gt; D. stramonium&lt;/em&gt; showed the presence of terpenoids and steroids. LC-MS analysis depicted presence of four major phytoconstituents in fraction-10 as daturaolone, daturadiol, stigmasterol and sitosterol with corresponding mass spectrum (m/z) of 440, 442, 412 and 414 respectively. &lt;strong&gt;Conclusion: &lt;/strong&gt;The present report concluded that active fraction-10 of&lt;em&gt; D. stramonium&lt;/em&gt; possesses potential immunostimulators that are capable of enhancing anticancer responses of human blood lymphocytes.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">435</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Aditi Gupta&lt;sup&gt;1&lt;/sup&gt;, Sunil Kumar&lt;sup&gt;2&lt;/sup&gt;, Neeraj Mahindroo&lt;sup&gt;2&lt;/sup&gt;, Reena Vohra Saini&lt;sup&gt;1*&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Animal Biotechnology Laboratory, Faculty of Applied Sciences and Biotechnology, Shoolini University, Himachal Pradesh, INDIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Pharmacology Laboratory, Faculty of Pharmaceutical Sciences, Shoolini University, Himachal Pradesh, INDIA.&lt;/p&gt;
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