<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Trisnawati Mundijo</style></author><author><style face="normal" font="default" size="100%">Yurnadi Hanafi Midoen</style></author><author><style face="normal" font="default" size="100%">Franciscus D. Suyatna</style></author><author><style face="normal" font="default" size="100%">Agung Eru Wibowo</style></author><author><style face="normal" font="default" size="100%">Kusmardi Kusmardi</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Effect of Seahorse Extract (Hippocampus comes L.) on Caspase-3 and TUNEL assay in Rats After Depot Medroxyprogesterone Acetate Induction</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Apoptotic</style></keyword><keyword><style  face="normal" font="default" size="100%">Caspase-3</style></keyword><keyword><style  face="normal" font="default" size="100%">DMPA</style></keyword><keyword><style  face="normal" font="default" size="100%">Seahorse</style></keyword><keyword><style  face="normal" font="default" size="100%">TUNEL assay</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">August 2022</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">253-258</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;Seahorse (&lt;em&gt;Hippocampus spp&lt;/em&gt;) is marine fish and have pharmacological activity, such as an antiinflammatory, antioxidative, antifatigue and improve the fertility. Depot medroxyprogesterone acetate (DMPA) is a contraception drug for male and affect the endocrine system by inhibiting pituitary gonadotropin with reduce testosterone levels in 12 weeks. There are limited studies reported the effects seahorse extract (SE) on Caspase-3 and TUNEL assay in rats induced by DMPA. Thirty &lt;em&gt;Sprague-Dawley &lt;/em&gt;(SD) male rats that were induced by 1.25mg/kgbw DMPA in 0 and 12 weeks. The animals were randomly into five groups, following: aquadest (G1), CMC 1% (G2), SE dose of 150 mg/kgbw (G3), SE dose of 225 mg/kgbw (G4), SE dose of 300 mg/kgbw (G5). The rats were gavage every day from seven until week eighteen. On the last week, we taken the right and left testis to observed the apoptotic on Caspase-3 and TUNEL assay. Apoptotic marker was observed through immunohistochemistry from testicular tissue and analysed with plugin ImageJ IHC profiler, which is H-score as the results. Data were analysed using One-Way ANOVA and Bonferroni’s post hoc tests. The SE decrease the Caspase-3 and TUNEL assay expression in rats induced by DMPA until eighteen weeks, with dose 150 mg/kgbw given the significant difference with p=0.028; &amp;lt;0.05 and p=0.000; &amp;lt;0.01. These results suggest that SE decreased germ cells apoptotic in DMPA induced rats.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article </style></work-type><accession-num><style face="normal" font="default" size="100%">01</style></accession-num><section><style face="normal" font="default" size="100%">253</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Trisnawati Mundijo&lt;sup&gt;1,2&lt;/sup&gt;, Yurnadi Hanafi Midoen&lt;sup&gt;3,*&lt;/sup&gt;, Franciscus D. Suyatna&lt;sup&gt;4&lt;/sup&gt;, Agung Eru Wibowo&lt;sup&gt;5&lt;/sup&gt;, Kusmardi Kusmardi,&lt;sup&gt;1,6-8&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Doctoral Programme Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Jl. Salemba Raya No.6, Jakarta, 10430, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Medical Biology, Faculty of Medicine, Universitas Muhammadiyah Palembang, Jl. KH Bhalqi No. 13 Ulu, Palembang, 30263, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, 10430, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, 10430, Indonesia.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;National Research and Innovation Agency, Indonesia, Puspiptek Street, Serpong, 15314, South Tangerang, Banten, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;6&lt;/sup&gt;Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.6, Jakarta, 10430, Indonesia.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;7&lt;/sup&gt;Drug Development Research Cluster, Indonesia Medical Educational and Research Institute, Jl. Salemba Raya No.6, Jakarta 10340, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;8&lt;/sup&gt;Human Cancer Research Cluster, Indonesia Medical Educational and Research Institute, Jl. Salemba Raya No.6, Jakarta 10340, INDONESIA.&lt;/p&gt;
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