<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Selvia Wiliantari</style></author><author><style face="normal" font="default" size="100%">Raditya Iswandana</style></author><author><style face="normal" font="default" size="100%">Berna Elya</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Total Polyphenols, Total Flavonoids, Antioxidant Activity and Inhibition of Tyrosinase Enzymes from Extract and Fraction of Passiflora ligularis Juss</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antioxidant</style></keyword><keyword><style  face="normal" font="default" size="100%">Passiflora ligularis Juss</style></keyword><keyword><style  face="normal" font="default" size="100%">Sweet granadilla.</style></keyword><keyword><style  face="normal" font="default" size="100%">Tyrosinase</style></keyword><keyword><style  face="normal" font="default" size="100%">Ultrasound-assisted extraction (UAE)</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2022</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">672-680</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background&lt;/strong&gt;: Sweet granadilla (&lt;em&gt;Passiflora ligularis Juss&lt;/em&gt;) grows in the cool highlands of Indonesia, one of which is the province of West Sumatera. Sweet granadilla has potent antioxidant activity and can inhibit the tyrosinase enzyme.&lt;strong&gt; Objective&lt;/strong&gt;: This study was performed to determine content of total polyphenols, total flavonoids, antioxidant activity, tyrosinase inhibition in different part of&lt;em&gt; P. ligularis&lt;/em&gt; extract and fraction.&lt;strong&gt; Materials and Methods:&lt;/strong&gt; Leaves, stems, peels and seeds&lt;em&gt; P. ligularis&lt;/em&gt; were separately extracted by the ultrasound-assisted extraction (UAE) method using 70% ethanol. Then, the ethanol extract was fractionated using n-hexane, ethyl acetate and distilled water. The ethanol extract and active fraction were determining antioxidant activity using FRAP and DPPH method, inhibition of tyrosinase enzyme, total polyphenol and total flavonoid content. This study was equipped with analysis of light microscopy, SEM microscopy and LC-MS. &lt;strong&gt;Results&lt;/strong&gt;: The highest total polyphenol content was found in the seed extract 176.22 ± 1.51 mg GAE/g extract and total flavonoid content was found in leaves extract 5.77 ± 0.48 mg QE/g extract. The highest antioxidant activity by FRAP method was found in seeds extract 80.79 ± 1.29 g Fe2SO4 equivalent/100 g extract and DPPH method was found in stem extract with IC&lt;sub&gt;50&lt;/sub&gt; value 9.00 ± 0.09. The highest percentage of tyrosinase inhibition (1 mg/ml) was found in seed extract 52.4 ± 2,55 %. In fraction of seed extract show that ethyl acetate fraction most active than others. &lt;strong&gt;Conclusion: &lt;/strong&gt;These results indicate that ethyl acetate fraction of seed&lt;em&gt; P. ligularis&lt;/em&gt; has potent antioxidants and good inhibition of the tyrosinase enzyme.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><accession-num><style face="normal" font="default" size="100%">26</style></accession-num><section><style face="normal" font="default" size="100%">672</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Selvia Wiliantari&lt;sup&gt;1&lt;/sup&gt;, Raditya Iswandana&lt;sup&gt;2&lt;/sup&gt;, Berna Elya&lt;sup&gt;1,*&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmacy Universitas Indonesia, Depok 16424, West Java, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Laboratory of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy Universitas Indonesia, Depok 16424, West Java, INDONESIA.&lt;/p&gt;
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