<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Nilda Lely</style></author><author><style face="normal" font="default" size="100%">Helmi Arifin</style></author><author><style face="normal" font="default" size="100%">Yufri Aldi</style></author><author><style face="normal" font="default" size="100%">Fatma Sri Wahyuni</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Anti-Inflammatory Effects of Methanol Extract, Hexane, Ethyl Acetate, and Butanol Fraction of Piper crocatum Ruiz &amp; Pav Leaves on Lipopolysaccharide-induced RAW 264.7 Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Anti-inflammatory Effect</style></keyword><keyword><style  face="normal" font="default" size="100%">LPS</style></keyword><keyword><style  face="normal" font="default" size="100%">Nitric oxide</style></keyword><keyword><style  face="normal" font="default" size="100%">Piper crocatum</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">November 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">1341-1346</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;em&gt;Piper crocatum&lt;/em&gt; Ruiz &amp;amp; Pav is a traditional Indonesian plant that is empirically used to treat various diseases. Several studies have stated that &lt;em&gt;Piper crocatum&lt;/em&gt; leaves extract has anti-inflammatory effects.&lt;em&gt; Piper crocatum &lt;/em&gt;leaves contain different secondary metabolites alkaloids, flavonoids, tannin-polyphenols, steroids, terpenoids, and saponins. The purpose of this study was to determine the anti-inflammatory effect of &lt;em&gt;Piper crocatum &lt;/em&gt;leaves extract and fraction on the production of nitric oxide in lipopolysaccharideinduced RAW 264.7 cells. Anti-inflammatory effect of methanol extract of&lt;em&gt; Piper crocatum&lt;/em&gt; leaves (MEPC), n-hexane fraction of &lt;em&gt;Piper crocatum &lt;/em&gt;leaves (HFPC), ethyl acetate fraction of &lt;em&gt;Piper crocatum &lt;/em&gt;leaves (EAFPC), and n-butanol fraction of &lt;em&gt;Piper crocatum&lt;/em&gt; leaves (BFPC) against RAW 264 cell macrophages, 7 Lipopolysaccharide-induced (LPS). Extraction of &lt;em&gt;Piper crocatum &lt;/em&gt;leaves was the maceration method. Cell viability was determined by the MTT method in the concentration range of 12.5;25;50;100;200 ug/ mL. The anti-inflammatory effects of MEPC, HFPC, EAFPC and BFPC were tested against nitric oxide (NO) production inhibition in LPS-induced RAW 264.7 macrophage cells. NO levels were determined by the NO Colorimetric Assay Kit as measured by the ELISA plate reader. The viability test of MEPC, HFPC, EAFPC, and BFPC from &lt;em&gt;Piper crocatum&lt;/em&gt; leaves showed the concentrations that gave the percentage of viability above 80% were concentrations of 100, 50, and 25 g/mL. MEPC, HFPC, EAFPC, and BFPC at concentrations of 100, 50, 25 μg/mL could significantly inhibit NO production with p&amp;lt;0.05 (p=0.000). The highest NO production inhibition effect was on EAFPC. MEPC, HFPC, EAFPC, and BFPC have antiinflammatory effects, with the highest effect on EAFPC.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">1341</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Nilda Lely&lt;sup&gt;1,3&lt;/sup&gt;, Helmi Arifin2, Yufri Aldi&lt;sup&gt;2&lt;/sup&gt;, Fatma Sri Wahyuni&lt;sup&gt;2&lt;/sup&gt;,*&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Postgraduate Student, Faculty of Pharmacy, Universitas Andalas, Padang, Sumatera Barat 25163, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Faculty of Pharmacy, Universitas Andalas, Padang, Sumatera Barat 25163, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;STIFI Bhakti Pertiwi Palembang. Jl. Ariodillah III No.22A, RT.27/RW.9, 20 Ilir D. IV, Ilir Tim. I, Kota Palembang, Sumatera Selatan 30128, INDONESIA.&lt;/p&gt;
</style></auth-address></record></records></xml>