<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Senzosenkosi Surprise Mkhize</style></author><author><style face="normal" font="default" size="100%">Mthokozisi Blessing Cedric Simelane</style></author><author><style face="normal" font="default" size="100%">Nothando Lovedale Gasa</style></author><author><style face="normal" font="default" size="100%">Ofentse Jacob Pooe</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Evaluating the Antioxidant and Heavy Metal Content of Pleurotus ostreatus Mushrooms Cultivated using Sugar Cane Agro-Waste</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antioxidant</style></keyword><keyword><style  face="normal" font="default" size="100%">DPPH</style></keyword><keyword><style  face="normal" font="default" size="100%">Heavy metals</style></keyword><keyword><style  face="normal" font="default" size="100%">Mushrooms</style></keyword><keyword><style  face="normal" font="default" size="100%">Pleurotus ostreatus</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">July 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">844-852</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; &lt;em&gt;Pleurotus ostreatus&lt;/em&gt;, is one of the most cultivated mushrooms with great economic and medicinal value that can be easily grown on various bio-waste substrates. However, biosafety evaluations on these mushrooms are rarely conducted. Thus, we sought to evaluate the concentration or presence of Heavy metals in P. ostreatus mushrooms cultivated on agro-bio-waste products. Furthermore, the effect of adding agro wastes on wheat bran (WB) cultivated mushrooms was evaluated. &lt;strong&gt;Methods:&lt;/strong&gt; Mushrooms grown in sugar cane tops and bagasse were supplemented with varying levels of WB. Atomic absorption spectrophotometer was applied to evaluate the concentration of heavy metals in the substrates and within mushrooms. Furthermore, DPPH free radical scavenging activity was used to determine antioxidant activity of mushroom extracts. &lt;strong&gt;Results:&lt;/strong&gt; The transfer factor analysis (TF) showed that mushrooms have an affinity to absorb Zn, Cd, Cu and Cr from all tested substrates during cultivation (TF&amp;gt;1). The addition of WB supplement into substrates resulted into significant increase in mushroom yield. However, the increased addition of WB, inversely affected the DPPH scavenging activity of the&lt;em&gt; P.ostreatus&lt;/em&gt; methanolic extracts. &lt;strong&gt;Conclusion: &lt;/strong&gt;The bioabsorption of heavy metals by &lt;em&gt;P. ostreatus&lt;/em&gt; is depended on the metal type. Based on these findings, mushrooms grown on these agro-waste appear to be safe and potent scavenging ability against free radicals.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">844</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Senzosenkosi Surprise Mkhize&lt;sup&gt;1&lt;/sup&gt;, Mthokozisi Blessing Cedric Simelane&lt;sup&gt;2&lt;/sup&gt;, Nothando Lovedale Gasa&lt;sup&gt;1&lt;/sup&gt;, Ofentse Jacob Pooe&lt;sup&gt;1,&lt;/sup&gt;*&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Discipline of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban, 4000, SOUTH AFRICA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park, 2006, SOUTH AFRICA.&lt;/p&gt;
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