<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tutik Sri Wahyuni</style></author><author><style face="normal" font="default" size="100%">Adita Ayu Permanasari</style></author><author><style face="normal" font="default" size="100%">Lidya Tumewu</style></author><author><style face="normal" font="default" size="100%">Aty Widyawaruyanti</style></author><author><style face="normal" font="default" size="100%">Achmad Fuad Hafid</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Qualitative and Quantitative Analysis of 70% Ethanol Extract from Ruta angustifolia for Developing Anti-Hepatitis C Agents</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Hepatitis C Virus</style></keyword><keyword><style  face="normal" font="default" size="100%">Infectious disease</style></keyword><keyword><style  face="normal" font="default" size="100%">Medicinal plants</style></keyword><keyword><style  face="normal" font="default" size="100%">Medicine</style></keyword><keyword><style  face="normal" font="default" size="100%">Ruta angustifolia</style></keyword><keyword><style  face="normal" font="default" size="100%">Rutin</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May 2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">682-687</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;Medicinal plants are potential sources for drug candidates. It possesses with various metabolites which have many pharmacology effects. &lt;em&gt;Ruta angustifolia&lt;/em&gt; is one of medicinal plants that has been used traditionally for liver disease. Previous study it has been demonstrated to inhibit hepatitis C virus under in vitro cell culture. It decreased protein NS3 level and gave synergistic effect in combination with simeprevir and telaprevir. This plant provides a prospective candidate to develop as anti-HCV Objective: This study evaluates the phytochemistry screening for qualitative assay and determine the concentration of rutin as marker compound for developing &lt;em&gt;R. angustifolia&lt;/em&gt; extract as anti-HCV agent.&lt;strong&gt; Materials and Methods:&lt;/strong&gt; &lt;em&gt;R. angustifolia&lt;/em&gt; leaves were extracted with 70% of ethanol. Extract and rutin were analysis their anti-HCV activity by in vitro culture cells of Huh7it. The concentration of rutin was determine by TLC densitometry. &lt;strong&gt;Results:&lt;/strong&gt; The 70% ethanol extract of &lt;em&gt;R. angustifolia &lt;/em&gt;dan rutin exhibit anti-HCV activities with IC&lt;sub&gt;50&lt;/sub&gt; value of 2.9 ± 0.8 μg/ml and 28.1 ± 5.6 μg/ml, respectively. Screening phytochemistry demonstrated to contain flavonoid, terpenoid, alkaloid and polyphenols. TLC densitometry analysis yield the concentration of rutin in extract 0.06 %. &lt;strong&gt;Conclusion:&lt;/strong&gt; Extract of 70% ethanol of &lt;em&gt;R. angustifolia &lt;/em&gt;has a potential anti-HCV activity. Extract of &lt;em&gt;R. angustifolia&lt;/em&gt; may provide a good candidate for developing anti-HCV agents.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">682</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Tutik Sri Wahyuni&lt;sup&gt;1,2,&lt;/sup&gt;*, Adita Ayu Permanasari&lt;sup&gt;2&lt;/sup&gt;, Lidya Tumewu&lt;sup&gt;2&lt;/sup&gt;, Aty Widyawaruyanti&lt;sup&gt;1,2&lt;/sup&gt;, Achmad Fuad Hafid&lt;sup&gt;1,2&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmaceutical Science, Faculty of Pharmacy, Universitas Airlangga, Jl. Mulyorejo, Surabaya 60115, INDONESIA.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Center of Natural Product Medicine Research and Development, Institute of Tropical Disease, Universitas Airlangga, Jl. Mulyorejo, Surabaya 60115, INDONESIA.&lt;/p&gt;
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