<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Vijitra Luang-In</style></author><author><style face="normal" font="default" size="100%">Worachot Saengha</style></author><author><style face="normal" font="default" size="100%">Benjaporn Buranrat</style></author><author><style face="normal" font="default" size="100%">Anut Chantiratikul</style></author><author><style face="normal" font="default" size="100%">Nyuk Ling Ma</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cytotoxicity of Selenium-Enriched Chinese Kale (Brassica oleracea var. alboglabra L.) Seedlings Against Caco-2, MCF-7 and HepG2 Cancer Cells</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Caco-2</style></keyword><keyword><style  face="normal" font="default" size="100%">HepG2</style></keyword><keyword><style  face="normal" font="default" size="100%">Isothiocyanate</style></keyword><keyword><style  face="normal" font="default" size="100%">Kale</style></keyword><keyword><style  face="normal" font="default" size="100%">MCF-7</style></keyword><keyword><style  face="normal" font="default" size="100%">Selenium</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">674-681</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;The Selenium-enriched Chinese kale (&lt;em&gt;Brassica oleracea&lt;/em&gt; var. &lt;em&gt;alboglabra &lt;/em&gt;L.) seedlings (Se-KS) have been known for its antioxidant activities, however its cytotoxic effects on various cancer cells are yet to be reported. &lt;strong&gt;Objective: &lt;/strong&gt;The objective of this work was to study the cytotoxic effects of Se-KS on Caco-2, MCF-7 and HepG2 cancer cells. &lt;strong&gt;Materials and Methods: &lt;/strong&gt;Freeze-dried seedlings were ground and incubated in 0.1 M citrate phosphate buffer pH 7.0 for 1 h at 37°C and extracted with dichloromethane to obtain total isothiocyanate (ITC) content which was quantified using the 1,2-benzenedithiole (BDT)-based cyclocondensation assay. The extracts from fresh seedlings were used to determine the cytotoxic effect on Caco- 2, MCF-7 and HepG2 cancer cells. &lt;strong&gt;Results: &lt;/strong&gt;Se-KS was found to contain total ITC content at 1.02 mmol/100 g dry weight (DW) which was significantly lower than that of 7-day old broccoli microgreens (1.60 mmol/100 g DW) as reference Cruciferous vegetables. In addition, Se-KS extract exhibited cytotoxic effects in a dose- and time-dependent manners. The lowest IC&lt;sub&gt;50&lt;/sub&gt; value of 82.83 μg/mL at 72 h was derived from HepG2 cells and the highest IC&lt;sub&gt;50&lt;/sub&gt; value of 164.00 μg/mL at 72 h was from MCF-7 cells suggesting that the Se-KS extract was most effective against HepG2 cells. Cancer cells showed signs of apoptotic bodies over 72 h and DNA fragmentations at 24 h indicating that the Se-KS extract was able to induce apoptosis in cancer cells in addition to cytotoxic effect. &lt;strong&gt;Conclusion: &lt;/strong&gt;Thus, Se-KS could be a novel source of organo selenium with chemopreventive benefits for functional food development.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">674</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Vijitra Luang-In&lt;sup&gt;1,*&lt;/sup&gt;, Worachot Saengha&lt;sup&gt;1&lt;/sup&gt;, Benjaporn Buranrat&lt;sup&gt;2&lt;/sup&gt;, Anut Chantiratikul&lt;sup&gt;3&lt;/sup&gt;, Nyuk Ling Ma&lt;sup&gt;4 &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Natural Antioxidant Innovation Research Unit, Department of Biotechnology, Faculty of Technology, Mahasarakham University, Khamriang, Kantarawichai, Maha Sarakham 44150, THAILAND.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Faculty of Medicine, Mahasarakham University, Muang, Maha Sarakham 44000, THAILAND.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Animal Feed Resources and Animal Nutrition Research Unit, Division of Animal Science, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, THAILAND.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, MALAYSIA.&lt;/p&gt;
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