TY - JOUR T1 - In vitro Evaluation of Seaweed Gracilaria verrucosa for Cytotoxic Activity against Cervical HeLa Cells JF - Pharmacognosy Journal Y1 - 2018 A1 - Micheylla Kusumaning Dewi A1 - Ade Arsianti A1 - Cut Raisya Zahira Zagloel A1 - Yully Astika Nugrahayning Aziza A1 - Kartika Dwi Kurniasari A1 - Baiq Kirana Dyahningrum Mandasari A1 - Riathul Masita A1 - Futihati Ruhama Zulfa A1 - Norma Nur Azizah A1 - Rista Putrianingsih KW - Cytotoxicity KW - Gracillaria verrucosa KW - HeLa cervical cancer cells KW - IC50 value KW - MTT Assay AB -

Background: Seaweed macroalgae of Gracilaria verrucosa has been known to have a potent anticancer activity, however the cytotoxicity against cervical cancer has not been explored further. Objective: This study aims to utilize Indonesia’s marine resource which is focused on seaweed macroalgae G. verrucosa as a future anti-cervical cancer agent. Materials and Method: Seaweed G. verrucosa originated from Labuan Aji beach, Nusa Tenggara Barat, Indonesia, extracted, macerated, and fractionated into four organic solvents of different polarity, consisting of hexane, ethyl acetate, chloroform and ethanol. Then, the macroalgae extracts are diluted into 8 different concentrations. Afterwards, in vitro anticancer activity evaluation of hexane, ethyl acetate, chloroform and ethanol extracts of G. verrucosa against cervical HeLa cells were conducted by MTT cell proliferation assay. Triplo mechanism is also applied in this study to increase the accuracy of the results. The anticancer activity is measured using IC50 value. Results: The four concentrated extracts G. verrucosa showed cytotoxicity against cervical HeLa cells. The greatest anticancer activity is depicted by hexane extract with an IC50 of 14.94 μg/mL, followed by chloroform (IC50 15.74 μg/mL), ethyl acetate (IC50 16.18 μg/mL), and ethanol (IC50 19.43 μg/mL). Conclusion: Our results clearly indicate that hexane, ethanol, chloroform, and ethyl acetate extracts of seaweed G. verrucosa can be further developed to be anti-cervical cancer agents, with hexane extract displaying the greatest cytotoxic effect.

VL - 10 IS - 5 ER -