TY - JOUR T1 - Antioxidant Activity of DPPH, CUPRAC, and FRAP Methods, as well as Activity of Alpha-Glucosidase Inhibiting Enzymes from Tinospora crispa (L.) Stem Ultrasonic Extract JF - Pharmacognosy Journal Y1 - 2022 A1 - Candra Irawan A1 - Imalia Dwi Putri A1 - Maman Sukiman A1 - Andita Utami A1 - Ismail A1 - Ratna Komala Putri A1 - Anisa Lisandi A1 - Andrean Nur Pratama KW - Antidiabetic KW - Antioxidant KW - Tinospora crispa (L.) KW - Ultrasound-assisted extraction. AB -

Aims: The goal of this work was to get the stem extract of Tinospora crispa (L.) using ultrasoundassisted extraction (UAE) with duration and amplitude changes to achieve optimal extraction conditions. The antioxidant potential of the extract as measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), CUPRAC (Cupric Ion Reducing Antioxidant Capacity), FRAP (Ferric Reducing Ability of Plasma), and its antidiabetic potential. Results: The third test of antioxidant methods showed that the measurement of antioxidant activity with, DPPH, CUPRAC, and FRAP gave significantly different results, but CUPRAC and FRAP gave the same response to the ethanol extract of Tinospora crispa stems, it can be concluded that the extract of Tinospora crispa stems in all treatments had strong antioxidant activity using either the CUPRAC or methods FRAP. In addition, it is very active as an antidiabetic by inhibiting alpha glucosidase. The optimum extract selected was extract B (extraction time was 35 minutes and amplitude 65%) with IC50 values for antioxidant activity in the CUPRAC method of 72.53 ± 0.18 mg/L and the FRAP method of 152.29 ± 2.16 mg/L, while the inhibition of alpha glucosidase was 0.30 ± 0.006 mg/L.

VL - 14 IS - 5 ER -