TY - JOUR T1 - Single Factor Effect of Natural Deep Eutectic Solvent Citric Acid-Glucose Based Microwave-Assisted Extraction on Total Polyphenols Content from Mitragyna speciosa Korth. Havil Leaves JF - Pharmacognosy Journal Y1 - 2021 A1 - Herman A1 - Arsyik Ibrahim A1 - Bakti Puji Rahayu A1 - M Arifuddin A1 - Yuspian Nur A1 - Wisnu Cahyo Prabowo A1 - Maryono A1 - Neneng Siti Silfi Ambarwati A1 - Laode Rijai A1 - Islamudin Ahmad KW - Citric acid-glucose KW - Microwave-assisted extraction KW - Mitragyna speciosa (Korth.) Havil KW - Natural deep eutectic solvent KW - Total polyphenols content AB -

Applying green chemistry principles in the exploration of an active secondary metabolite from natural products has continued to experience a significant increase in the last decade, mainly natural deep eutectic solvent (NADES) usage. Mitragyna speciosa (Korth.) Havil (Rubiaceae family) is a native of East Kalimantan, which is traditionally used as an herb to treat headaches and inflammation by drinking boiled water a few leaves. On the other hand, this plant has an addictive effect. This study aims to determine the single factor effect of NADES citric acid–glucose-based microwave-assisted extraction (MAE) to obtain the yield of total polyphenols content (TPC) from M. speciosa leaves. Dried powder of M. speciosa leaves was extracted using NADES citric acid-glucose based MAE method with some different conditions such as NADES (citric acid-glucose) ratio (4:1, 5:1, 6:1 g/g), solid-liquid ratio (10:1, 15:1, 20:1 mL/g), microwave power (90, 270, 450 Watts), and extraction time (15, 20, 25 min). The determination of TPC was performed using Folin-Ciocalteau reagent and gallic acid as a standard with different concentration levels. The absorbance measured using spectrophotometer UV-VIS at 746 nm to obtain a regression formula of Y = 0.0022X–0.00095, where R2 = 0.9977. Based on the result, the best of single factor condition effect was obtained the TPC value including NADES ratio of 248.69 mg GAE/g sample (5:1 g/g citric acid-glucose), a solid-liquid ratio of 146.93 mg GAE/g sample (15:1 mL/g solvent-sample), microwave power of 192.20 mg GAE/g sample (270 Watts), and extraction time of 358.59 mg GAE/g sample (15 min). The single factor effect of NADES citric acid-glucose-based MAE shows a difference in TPC value based on various conditions of this method.

VL - 13 IS - 5 ER -