TY - JOUR T1 - Isolation of flavonoid from Abies webbiana leaves and its activity JF - Pharmacognosy Journal Y1 - 2016 A1 - Dinesh Kumar Yadav A1 - Mohammed Ali A1 - Ashoke Kumar Ghosh A1 - Babita Kumar KW - 1-H NMR KW - Abies webbiana KW - CCl4. KW - Cisplatin KW - Quercetin AB -

Background: Abies webbiana commonly known as Talispatra in Bengali and Hindi, Talispatram in Sanskrit and Indian Silver Fir in English. This is a large, tall, evergreen tree occurring in the Himalayan region from Kashmir to Assam in India. It comes under the Family: Pinaceae. The present study was designed for isolation of flavonoid from ethyl acetate extract of A. webbiana leaves and assessed their toxic effect on liver and kidney. Materials and Methods: The isolation of flavonoid using different chromatographic methods (thin layer and column chromatography). The isolated flavonoid was identified; Structures and chemical bonds were analyzed by using MP, FTIR, 1-H NMR and MS spectral analysis. Effect of flavonoid on liver and kidney was assessed by inducing (0.1 ml/kg) CCl4 (i.p.) and (6 mg/kg) Cisplatin (i.p.) respectively measured by biochemical marker of liver and kidney. Results and Discussion: It was identified that isolated compound was as 4’-hydroxy quercetin on the basis of FTIR, 1-H NMR and MS spectral analysis. Isolated flavonoid reduced the increased biochemical marker (BM) of liver and kidney. The BM was increased by inducing CCl4 and Cisplatin respectively. Conclusion: Isolated compound was 4’-methoxy quercetin and significantly protect the liver and kidney.

VL - 8 IS - 4 ER -