TY - JOUR T1 - Inhibition on Urease and Thermal Induced Protein Denaturation of commonly used Antiulcer Herbal Products. Study based on in-vitro assays JF - Pharmacognosy Journal Y1 - 2015 A1 - Haroon Khan A1 - Murad Ali Khan A1 - Abdul Rauf A1 - Ashhad Haleemi A1 - Shivkanya Fuloria A1 - Neeraj Kumar Fuloria KW - Akseer ULCER KW - Antiurease activity KW - Endemali KW - Thermal induced protein denaturation. AB -

Background: In-vitro urease inhibitory and thermal induced protein denaturation inhibitory activitieswasperformed for two commonly used herbal productsEndemali and Akseer ULCER in the treatment of ulcers.Objectives: to evaluate the antiulcer potential of two commonly used herbal products, Endemali, Akseer ULCER. Material and Method: In urease inhibitory assay , enzyme solution, extract, diferentregaents added and absorbance was measured at 630 nm (50 min, pH 8.2) and thiourea used as standard. In protein denaturation assay the egg albumin was mixed with different concentration of test compounds, buffer absorbance was measured. Aspirin was used as standard. Results: The Endemalihad a profound effect on the urease activity in a concentration dependent manner with EC50 valueof 0.468 mg/ml. The Akseer ULCER antagonized the urease activity markedly with EC50 value of 0.374 mg/ml.These tested herbal products caused marked inhibition of thermal induced protein denaturation in a concentration dependent manner. The potency in the form of EC50 for Endemali, Akseer ULCER was measured as 323, 337 µg/mlrespectively. Conclusion: In short, the tested herbal drug showed strong inhibition on urease activity and inhibition on thermal induced protein denaturation thus our study validated their uses in the treatment of ulcers.

Key words: Akseer ULCER, Endemali, Antiurease activity, Thermal induced protein denaturation.

VL - 7 IS - 3 ER -