02092nas a2200205 4500008004100000245011400041210006900155260001200224300001000236490000600246520144300252653001901695653002501714653001401739653002001753653002401773100002401797700002701821856003801848 2016 eng d00aAcute and Sub-acute Toxicity Study of Aqueous Extracts of Enicostemma axillare (Lam.) Raynal in Animal models0 aAcute and Subacute Toxicity Study of Aqueous Extracts of Enicost c09/2015 a10-190 v83 a

Background: Enicostemma axillare (Lam.) Raynal is used in traditional practice for the treatment of diabetes, malaria and liver disorders. No systematic toxicity study was described for this plant and hence the present was undertaken to evaluate acute and sub-acute toxicity of aqueous extract of Enicostemma axillare (AEEA). Objective: The acute oral toxicity study of AEEA was carried out as per the OECD guidelines 423 in mice and the sub-acute toxicity was carried out as per the guidelines set by OECD 407 in male and female rats. Materials and Methods: Body weight, food and water consumption, hematological parameters, biochemical parameters, organ weight and histopathological analysis were carried out. Results: No gross toxicity and mortality was observed upto a dose of 2000 mg/kg. For sub-acute toxicity test, 200 mg/kg and 400 mg/kg daily dose of AEEA administered orally for 28 days in male and female group of rats not exhibited any signs of toxicity and mortality. Conclusion: In acute oral toxicity study, the oral administration of AEEA in mice was found to be safe up to a dose of 2000 mg/kg. Both male and female treated rats showed no change in hematological, biochemical and histological investigations and no signs of toxicity were observed upto the dose of 400 mg/kg in rats.

10aAcute toxicity10aEnicostemma axillare10aHistology10aOECD guidelines10aSub-acute toxicity.1 aRajasekaran, Aiyalu1 aArivukkarasu, Ramasamy uhttps://www.phcogj.com/article/99