02620nas a2200289 4500008004100000245013300041210006900174260001900243300001400262490000700276520174000283653002002023653001702043653001802060653001702078653001402095100002102109700002102130700001802151700002102169700002302190700002002213700002002233700002102253700001702274856003902291 2019 eng d00aPhytosomal Nanoparticles Preparation of Curcuminoids to Enhance Cellular Uptake of Curcuminoids on Breast Cancer Cell Line MCF-70 aPhytosomal Nanoparticles Preparation of Curcuminoids to Enhance  cSeptember 2019 a1037-10450 v113 a

Objective: Curcuminoids, the bioactive compounds extracted from Curcuma longa consisting of Curcumin (CUR), demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC), have shown promising biological effects, including anticancer activity. This study sought to prepare a physically stable phytosomal nanoparticles of curcuminoids (Curs-Phyto) to facilitate uptake of curcuminoids on breast cancer cells line, and further increase the cytotoxicity against cancer cells. Methods: The evaporation combined extrusion technique was employed to prepare phytosomal curcuminoids nanoparticles. The interaction between curcuminoids and phospholipid by a hydrogen bond was confirmed by differential scanning calorimetry (DSC), powder X-ray diffraction (XRD), fourier transform infrared (FT-IR), and 1H nuclear magnetic resonance (1H-NMR). Their physicochemical characterizations and stability in simulated gastric and intestinal media were investigated. The effects of Curs-Phyto on MCF-7 cells were evaluated by flow cytometry, MTS assay and cell cycle analysis. Results: We found that the Curs-Phyto were formed at a spherical shape with good size (~ 180 nm), a narrow size distribution (PDI < d0.2), high complexation rate (~ 87%, 95%, and 90% for BDMC, DMC, and CUR respectively) and high loading capacity of curcuminoids. More importantly, the Curs-Phyto showed the increased cellular uptake and enhanced cytotoxicity against MCF- 7 cancer cells, compared to free curcuminoids. Conclusion: These results indicated that the phytosome could be a promising oral delivery system for curcuminoids for cancer treatment.

10aCellular uptake10aCurcuminoids10aFACS analysis10aNanoparticle10aPhytosome1 aVan Long, Nguyen1 aHa, Bui, Thi Thu1 aTuan, Anh, Vu1 aVan Luong, Hoang1 aLinh, Nguyen, Tung1 aDuc, Thanh, Chu1 aDai, Phung, Cao1 aYong, Chul, Soon1 aVan Men, Chu uhttps://www.phcogj.com/article/987