02755nas a2200217 4500008004100000245010400041210006900145260001900214300001400233490000700247520204800254653003402302653002702336653001502363653001302378100003302391700002602424700002302450700002502473856003902498 2019 eng d00aQuantitative Analysis of Hispidulin Content in Clerodendrum petasites Roots Distributed in Thailand0 aQuantitative Analysis of Hispidulin Content in Clerodendrum peta cSeptember 2019 a1093-10990 v113 a

Introduction: Clerodendrum petasites (Lour.) S. Moore (locally known as Mai-Thao-Yaai-Mom), belonging to the Verbenaceae family, is widely formulated into multi-herb remedy, Ben-Cha- Lo-Ka-Wi-Chian remedy, possessing antipyretic activity. C. petasites exhibits many biological activities, such as antioxidant, anti-inflammatory, antipyretic, etc. The flavonoid hispidulin is one of the main active compounds present in C. petasites, containing anti-atheromatous, antitumor and antispasmodic effects. Objective: The present study aimed to determine the hispidulin content in the dried roots of C. petasites using HPLC technique. Methods: C. petasites dried roots, collected from twelve different areas, were extracted with ethanol using Soxhlet apparatus, and then subjected to HPLC-PDA to quantify hispidulin content. The quantitative method using HPLC-PDA technique was validated. Results: The optimized HPLC coupling with PDA detector (HPLC-PDA) was validated for the quantitative analysis of hispidulin content in C. petasites roots in terms of linearity (y = 210,200,536.6667x – 448,756.2667; R2 = 0.9997), accuracy (88.82-107.69% recovery), precision (0.66% RSD for repeatability precision; 1.17% RSD for intermediate precision), limit of detection (2.30 μg/mL), limit of quantitation (7.00 μg/mL), specificity (peak purity index = 1.0000) and robustness (% RSD < 1). The amount of hispidulin content in the extracts of C. petasites roots conducted from the validated method was found to be 0.0182 ± 0.0109 g/100 g crude drug. Conclusion: The HPLC-PDA analysis was able to effectively determine hispidulin in C. petasites roots. The hispidulin contents in C. petasites dried roots from various areas in Thailand were revealed which could be used for the specification of this crude drug with reference to its chemical marker.

10aBen-Cha-Lo-Ka-Wi-Chian remedy10aClerodendrum petasites10ahispidulin10aHPLC-PDA1 aTangsongcharoen, Thanyathorn1 aIssaravanich, Somchai1 aPalanuvej, Chanida1 aRuangrungsi, Nijsiri uhttps://www.phcogj.com/article/964