02148nas a2200205 4500008004100000245011400041210006900155260001900224300001200243490000700255520149600262653002701758653001901785653002001804653002201824653001401846100002201860700002101882856003901903 2019 eng d00aCo-Chemotherapeutic Effect of Ageratum conyzoides L. Chloroform Fraction and 5-Fluorouracil on Hela Cell Line0 aCoChemotherapeutic Effect of Ageratum conyzoides L Chloroform Fr cSeptember 2019 a913-9180 v113 a

Objective: This study was to determine the co-chemotherapeutic effect of the chloroform fraction of bandotan (Ageratum conyzoides L.) (CFB) and its combination with 5-Fluorouracil (5-FU) by in vitro and in silico assay. Methods: Ethanolic extract of bandotan were fractionated with chloroform. Thin Layer Chromatography (TLC) used to identify active compound and in vitro study with MTT Assay to determine the viability of HeLa cells after extract treatment. Molecular docking used Autodock Vina for in silico study to visualize molecular interaction and affinity between nobiletin and 5-FU with Bcl-XL protein. Results: The result of TLC for CFB showed the Rf value of 0.75, it has the similar value with quersetin standard and indicated that CFB contains flavonoid compound. The Molecular docking had ΔG for nobiletin and 5-FU were -8.0 and -4.7 kcal/mol, respectively. This result showed that the affinity of nobiletin with Bcl-XL protein higher than 5-FU. Single cytotoxic assay of CFB and 5-FU showed the IC50 value of 30 μg/ml and 45 μg/ml, respectively. Combination assay of CFB and 5-FU showed the CI value of 0.36, meaning the presence of synergistic effects. Conclusion: CFB has a positive effect to inhibit viability of HeLa cervical cancer cells and potential to develop as co-chemotherapy agent with 5-FU.

10aAgeratum conyzoides L.10aBcl-XL protein10aCytotoxic assay10aMolecular docking10aNobiletin1 aFebriansah, Rifki1 aKomalasari, Titi uhttps://www.phcogj.com/article/953