02107nas a2200217 4500008004100000245009100041210006900132260001900201300001200220490000700232520147400239653001101713653001001724653001901734653002001753653001401773653001501787100002601802700002201828856003901850 2019 eng d00aGlucosinolates, Degradation Products and Myrosinase Activity in Raphanus sativus Linn.0 aGlucosinolates Degradation Products and Myrosinase Activity in R cSeptember 2019 a866-8720 v113 a

This research was conducted to assess the gluocosinolate (GSL), isothiocyanate (ITC) and myrosinase content in two cultivars of Raphanus sativus Linn. (white and red) roots. LC-ESIMS investigation was done on desulfated GSLs since this methodology has been previously established for efficient GSL analyses. The major GSLs: sinigrin (1) 1,2-dihydroxy-2-phenylethyl glucosinolate (2), 4-hydroxyglucobrassicin (3), glucoraphasatin (4) and 4-methoxyglucobrassicin (5) were found in red radishes; whereas, only 1 and 4 were obtained in white radishes. Myrosinase was analyzed in the tubers due to its ability to catalyze and hydrolyze GSLs into ITCs. This β-thioglucosidase enzyme was found to be over 10 times more active in red tubers (2.05E-02 units) than in white radishes (1.55E-03 units) and the results were linked to the presence/absence of the outer covering of the tubers. Due to the promising medicinal properties of the aglucone derivative of compound 4, 4-methylthio-3-butenyl isothiocyanate (6), the ITC analog was monitored using gas chromatographic mass spectral analyses after myrosinase-mediated hydrolysis. From the results, it can be construed that the occurrence of GSLs 1-5 and the bioactive agent 6 were inherent in the R. sativus cultivars evaluated.

10aGC-FID10aGC-MS10aGlucosinolates10aIsothiocyanates10aLC-ESI-MS10aMyrosinase1 aTan, Maria, Carmen S.1 aNoel, Marissa, G. uhttps://www.phcogj.com/article/945