02289nas a2200217 4500008004100000245013400041210006900175260001400244300001200258490000700270520161300277653001501890653001101905653002701916653002301943653002001966100002501986700001602011700000502027856003902032 2019 eng d00aDetermination of Specific and Non-Specific Parameters of Simplicia and Ethanolic 70% Extract of Gadung Tubers (Dioscorea hispida)0 aDetermination of Specific and NonSpecific Parameters of Simplici cJuly 2019 a759-7630 v113 a

Background: D. hispida can be used as a painkiller, rheumatic drugs and antioxidant. Based on its medicinal properties, D. hispida can be used as a traditional medicine that must be guaranteed on quality, safety and benefit. Therefore, standardization is needed. This aim of this study was to obtain some specific and non-specific parameter of simplicia and ethanolic 70% extract of D. hispida tubers from three different regions. Materials and Methods: The samples were macerated by using ethanol 70%. Results: The results showed that the specific parameters of D. hispida simplicia; the total water soluble extract was 11.25-16.20%; the total ethanol soluble extract was 6.42-9.39%; the chromatogram profile was obtained by using thin layer chromatography in toluene-ethyl acetate-chloroform (5:1:4) mobile phase with β–sitosterol as standard, the total phenolic content was 2.15–2.50 expressed in mgGAE/g simplicia. The non-specific parameters of D. hispida simplicia; the total loss on drying was 10.53-12.40%; the total ash content was 5.81-5.94%; the total acid insoluble ash content was 0.20-0.22%. The specific parameters of D. hispida extract; the total phenolic content was 10.30-11.72 expressed in mgGAE/g extract. The non-specific parameters of D. hispida extract; the total water content was 10.27-10.47%; the total ash content was 2.84-2.93%; the total acid insoluble ash content was 0.14-0.19. Conclusion: conform the parameters.

10aD. hispida10aGadung10aNon-specific parameter10aSpecific parameter10astandardization1 aSari, Sasmita, Retno1 aElya, Berna1 a uhttps://www.phcogj.com/article/928