02476nas a2200265 4500008004100000245013300041210006900174260001400243300001200257490000700269520163800276653001601914653002001930653001301950653002501963653000901988100001701997700001902014700002702033700001902060700003202079700003502111700002502146856003902171 2019 eng d00aThe Evaluation of Dietary Black Soybean and Purple Sweet Potato on Insulin Sensitivity in Streptozotocin - Induced Diabetic Rats0 aEvaluation of Dietary Black Soybean and Purple Sweet Potato on I cJuly 2019 a639-6460 v113 a

Introduction: Hyperglycemia mediates the production of excess free radicals and reduces endogenous antioxidant in type 2 diabetes mellitus (T2DM). Black soybean (BSB) is rich in antioxidant mainly from isoflavones, whereas the antioxidant of purple sweet potato (PSP) comes from high anthocyanin. The study aimed to evaluate the efficacy of BSB, PSP, and its combination on malondialdehyde (MDA), superoxide dismutase (SOD) concentrations, insulin and insulin receptor substrate-1 (IRS-1) expression in T2DM rats. Methods: T2DM induced by high-calorie diet for five weeks and then injected with a low dose of streptozotocin (30 mg/kg BW) intraperitoneally. The DM rats then treated with black soybean (DM + BSB), purple sweet potato (DM + PSP) and the combination of BSB and PSP 1:3, 2:2, 3:1 respectively (DM + C1- 3). Treatments were given for thirty days. The effect of BSB, PSP and its combination evaluated by measuring SOD and MDA, necrosis in pancreas evaluated through hematoxylin-eosin (HE) and insulin and IRS-1 expression in pancreas through immunohistochemistry-fluorescence (IHC-F). Results: Our result indicated that there were no significant differences of BSB and the combination in decreasing MDA concentrations. The ratio of BSB and PSP combination of 2:2 increase SOD towards near normal, decrease necrosis, and improve insulin and IRS-1. Conclusion: The combination of BSB and PSP had the potential to improve insulin sensitivity through the increase of SOD, reduce necrosis, and improve insulin and IRS-1 expression.

10aAntioxidant10aMalondialdehyde10aNecrosis10aSuperoxide dismutase10aT2DM1 aGofur, Abdul1 aWitjoro, Agung1 aNingtiyas, Erni, Widya1 aSetyowati, Evi1 aMukharromah, Siti, Aminatul1 aAtho’illah, Mochammad, Fitri1 aLestari, Sri, Rahayu uhttps://www.phcogj.com/article/899