02654nas a2200229 4500008004100000245016000041210006900201260001500270300001200285490000700297520184500304653001602149653003402165653001702199653002802216653003302244100003402277700002402311700003002335700002002365856003902385 2019 eng d00aXanthones Analysis and Antioxidant Activity Analysis (Applying ESR) of Six Different Maturity Levels of Mangosteen Rind Extract (Garcinia mangostana Linn.)0 aXanthones Analysis and Antioxidant Activity Analysis Applying ES cMarch 2019 a369-3730 v113 a

Background: Mangosteen (Garcinia mangostana Linn.) is tropical fruit that known to contain bioactive compounds which has particular function as antioxidants, anti-inflammatory and antibacterial. Mangosteen rind extract is known to have high contents of xanthone compounds which have antioxidant and free radical scavenging activities. This study was aiming to determine the contents of xanthone derivatives using Liquid Chromatography Mass Spectrometry (LC-MS/MS) and antioxidant activity of six different maturity levels of mangosteen rind extract using electron spin resonance (ESR). Method: Mangosteen was classified based on the maturity level by its visual characteristic and divided into six criteria of maturity. The mangosteen rinds were extracted through maceration and freeze drying method. Qualitative analysis of xanthone was obtained using LC-MS/MS. Antioxidant activity was determined using ESR spectrometer. To evaluate antioxidant activity, using 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used as free radical scavenger method. Results: The LC-MS/MS analysis showed that the highest concentrations are filled by mangosteen rind with maturity level 6. Moreover, each maturity levels of mangosteen rind extract has different g value compared to DPPH free radical. Different g value indicated the different type of free radical and decreasing g value indicated the declining intensity of free radical. The higher decreasing of g value was mangosteen rind with maturity level 4, 5 and 6. Conclusion: Mangosteen rind extract contains xanthone and its derivatives and ESR analysis showed that different maturity levels on mangosteen rind has different antioxidant activity as indicated by declining free radical intensity.

10aAntioxidant10aElectron spin resonance (ESR)10aFree radical10aMangosteen rind extract10aXanthone Garcinia mangostana1 aGondokesumo, Marisca, Evalina1 aPardjianto, Bambang1 aSumitro, Sutiman, Bambang1 aWidowati, Wahyu uhttps://www.phcogj.com/article/842