02866nas a2200253 4500008004100000245014000041210006900181260001200250300001200262490000600274520200200280653002402282653004602306653001502352653002002367653002702387100002802414700002802442700002402470700002702494700002602521700002702547856003802574 2015 eng d00aAntibacterial Activity of Tinctures from Tree leaves belonging to the Bignoniaceae family and their Synergistic Effect with Antibiotics0 aAntibacterial Activity of Tinctures from Tree leaves belonging t c01/2015 a400-4050 v73 a

Context: Some species of Bignoniaceae are widely used in medicinal practice by the natives of South America. Aims: Tinctures and infusions from twelve tree species of this family were evaluated for in vitro antibacterial activity against pathogenic bacteria. The effect of interactions between the four most active extracts and conventional antibiotics was also evaluated. Methods and Material: Bioautography and disc diffusion methods were used to select the most active extracts, then agar macrodilution and broth microdilution method were used to determine the minimal inhibitory and minimal bactericidal concentration (MIC and MBC). Time-kill assay and checkerboard method were employed to determine the type of antimicrobial effect and synergism, respectively. Results: It could be determined that tinctures from Catalpa bignonioides, Handroanthus pulcherrimus, Tabebuia nodosa and Tecoma stans were able to inhibit bacterial growth. The MIC and MBC observed were between 125-1000 μg GAE/ ml and 500-1000 μg GAE/ml, respectively. The tested extracts were more effective against Gram-positive microorganisms. Time-kill experiments indicated bacteriostatic activity. Phytochemical screening showed terpenoids, phenols and flavonoids. Alkaloids were detected only in Tecoma stans. Among these combinations, the best was Tabebuia nodosa extract plus gentamicin. In most cases, MIC values were reduced 16-32 times for antibiotics, and even 8-16 times for extracts. Conclusion: These results revealed that some of the selected combinations could efficiently inhibit the growth of tested strains at lower concentrations than those required for the lonely use of the antimicrobial. These extracts would improve the efficacy of antibiotics against resistant bacteria, hence they could be used for anti-infective therapy.

10aCheckerboard method10aFractional inhibitory concentration (FIC)10aGentamicin10aTabebuia nodosa10aTime-kill experiments.1 aAnalía, Torres, Carola1 aBeatriz, Nuñez, María1 aInés, Isla, María1 aPaola, Castro, Marcela1 aMaría, Gonzalez, Ana1 aCatiana, Zampini, Iris uhttps://www.phcogj.com/article/84