02000nas a2200229 4500008004100000245012500041210006900166260001700235300001200252490000600264520129700270653001601567653001701583653001701600653002301617653001401640653001001654100002401664700002601688700001801714856003801732 2015 eng d00aCytotoxicity Study of Ethanol Extract of the Leaves of Asam Kandis (Garcinia cowa Roxb.) on T47D Breast Cancer Cell line0 aCytotoxicity Study of Ethanol Extract of the Leaves of Asam Kand cNov-Dec 2015 a369-3710 v73 a

Objective: To investigate the cytotoxic effect of ethanolic extract of the leaves of asam kandis (Garcinia cowa Roxb.) against T47D breast cancer cells. Methods: The cytotoxicity of ethanol extract was carried out by measuring the activity of mitochondrial dehydrogenase in living cells that have ability to convert dissolved MTT pale yellow to purple formazan product. The extract was added at various concentrations (0.1, 1, 10 and 100 μg/mL). The level of cytotoxicity was determined by calculating the IC50 value that was based on the percentage of the cell death after 24 hours treatment with the extract. Cell morphological changes were observed by using inverted microscope. Results: The IC50 value showed that ethanol extract of leaves of asam kandis could resist T47D breast cancer cells with IC50 6.13 ± 3.51 μg/mL. The statistic results proved that ethanol extract of the leaves of asam kandis could inhibit the growth of T47D breast cancer cells significantly at concentrations of 10 μg/mL and 100 μg/mL. Conclusion: The results suggest that ethanol extract of the leaves of asam kandis was potential source of herbal medicine for cancer-related ailments.

10aAsam kandis10aBeast cancer10aCytotoxicity10aGarcinia cowa Roxb10aMTT Assay10aT47D.1 aWahyuni, Fatma, Sri1 aTriastuti, Dini, Hara1 aArifin, Helmi uhttps://www.phcogj.com/article/82