02488nas a2200253 4500008004100000245012700041210006900168260001700237300001000254490000700264520169100271653000901962653003101971653002402002653002602026653001402052100001802066700002602084700001902110700002202129700002302151700002102174856003902195 2019 eng d00aThe Anti-proliferative and Anti-bacterial Activity of Argan oil and Crude Saponin Extract from Argania spinosa (L.) Skeels0 aAntiproliferative and Antibacterial Activity of Argan oil and Cr cJanuary 2019 a26-310 v113 a

Introduction: Argan oil is a well-known cosmeceutical that is commercially available. It is traditionally used for the treatment of acne and skin inflammation among others. The objective of this study was to assess the anti-proliferative and antibacterial activities of argan oil and a crude saponin extract from the argan tree (Argania spinosa (L.) Skeels) that is endemic to Morocco. Materials and Methods: The anti-proliferative activity of argan oil and the crude saponin extract was assessed by the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium- 5-carboxanilide (XTT) assay on A431; HaCat; HeLa; MCF-7 and UCT-Mel 1 cells. The antibacterial activity was evaluated by the broth microdilution method against two species of bacteria: Cutibacterium acnes and Prevotella intermedia. Results: The results of this study indicated that the argan oil sample did not inhibit the cell growth of the specified cell lines up to 1000μg/ml, while the crude saponin extract had low anti-proliferative activity. The minimal inhibitory concentration (MIC) values for both the argan oil and the crude saponin extract were found to be 500μg/ml against Cutibacterium acnes. No antibacterial activity from the argan oil or the crude saponin extract was evident against Prevotella intermedia up to a concentration of 12.5mg/ml. Conclusion: The results of this study indicated that argan oil and the crude saponin extract might have direct inhibitory effects on the growth and proliferation of Cutibacterium acnes. This finding supports the use argan oil as a treatment for acne vulgaris.

10aAcne10aBroth microdilution method10aCutibacterium acnes10aPrevotella intermedia10aXTT assay1 aLall, Namrita1 aDe Canha, Marco, Nuno1 aSzuman, Karina1 aCharrouf, Zoubida1 aDavids, Lester, M.1 aRademan, Sunelle uhttps://www.phcogj.com/article/801