03188nas a2200325 4500008004100000245011800041210006900159260001700228300001000245490000700255520203800262653009602300100003502396700003402431700002602465700002402491700000502515700002702520700003002547700003202577700000502609700003002614700003302644700003002677700003302707700001902740700003102759700003302790856003902823 2019 eng d00aAntioxidant and Anti-inflammatory Activities of Bauhinia ungulata L. (Fabaceae) on LPS-Stimulated RAW 264.7 Cells0 aAntioxidant and Antiinflammatory Activities of Bauhinia ungulata cJanuary 2019 a37-420 v113 a

Objective: The present study aimed to investigate the antioxidant, immunomodulatory and antimicrobial activities of Bauhinia ungulata L. Method: A flavonoid-rich fraction was obtained from the Bauhinia ungulata L stem, called the ethyl acetate fraction of Bauhinia ungulata (FABU). The total antioxidant capacity of the FABU was determined through the phosphomolibdenium reduction method. For the evaluation of its antioxidant activity on a cell culture model, LPS-stimulated RAW 264.7 cells were treated with different concentrations of FABU and the reactive oxygen species (ROS), nitric oxide (NO), hydrogen peroxide (H2O2) and thiobarbituric acid reactive substances (TBARS) production levels were measured. For the analysis of its immunomodulatory capacity, TNF-α, TGF-β and IL-10 levels were determined in the culture supernatant. In order to determinate the antimicrobial activity of FABU, antifungal and antibacterial susceptibility testing was performed against Candida albicans, methicillinsensitive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa strains. Result: The FABU demonstrated neither significant antimicrobial activity nor immunodulatory capacity; on the other hand, its potential antioxidant activity was demonstrated by the phosphomolibdenium reduction assay. Also, FABU treatment inhibited the ROS, NO, H2O2 and TBARS levels in the supernatant of LPS-stimulated cells. Conclusion: A significant reduction in the amount of reactive oxygen and nitrogen species (RONS) was observed, in addition to lipid peroxidation inhibition. Our data suggest that the FABU is a natural antioxidant complex that may interfere in the cascade of cell damage caused by free radicals and a promising potential drug in chronic disease models in which immunopathogenicity involves high levels of RONS.

10aBauhinia ungulata; RAW 264.7 cells; Lipopolysaccharide; Free radicals; Antioxidant activity1 aRodrigues, Raphael, de Oliveir1 aYaochite, Juliana, Navarro Ue1 aBraga, Milena, Aguiar1 ade, Amanda, Ribeiro1 a1 aSasahara, Greyce, Luri1 aFonseca, Said, Gonçalves1 aAraújo, Thiago, Dias de Va1 a1 aSantiago, Maria, Pinheiro1 ade Sousa, Leôncio, Mesquita1 ade Carvalho, Jarbas, Lima1 aAires, Francisca, Bruna Stef1 aNascimento, do1 aJúnior, Hélio, Vitoriano1 aNagao-Dias, Aparecida, Tiemi uhttps://www.phcogj.com/article/787