03189nas a2200289 4500008004100000245020400041210006900245260001700314300001100331490000700342520223800349653001802587653001602605653002202621653001102643653001102654653002502665100002802690700001702718700002002735700001802755700002502773700002402798700002302822700001502845856003902860 2019 eng d00aSequential Fractionation by Organic Solvents Enhances the Antioxidant and Antibacterial Activity of Ethanolic Extracts of Fruits and Leaves of Terminalia bellerica from North Western Himalayas, India0 aSequential Fractionation by Organic Solvents Enhances the Antiox cJanuary 2019 a94-1010 v113 a

Background: Terminalia bellerica belonging to family combretaceae is one of the major components of “Triphala”, an Ayurvedic formulation. Objective: To evaluate antioxidant potential in the ethanolic extract and its active fraction (chloroform, ethyl acetate, n- butanol and aqueous fraction) from fruits and leaves of Terminalia bellerica. Materials and Methods: Folin-Ciocalteau method and aluminium chloride method was used to quantify total phenolic and flavonoid content respectively in ethanolic extract and their fractions from fruits and leaves of T. bellerica. The antioxidant activity was evaluated using total antioxidant activity, DPPH, FRAP and total antioxidant activity methods. Results: Total phenolics (254.72±3.03 mg/g GAE and 227.52±1.38 mg/g GAE) and flavonoids (64.77±1.24 mg/gm RE and 75.57±1.38 mg/gm RE) content was higher in ethyl acetate fraction of both fruits and leaves sample of T. bellerica, respectively. The order of free radical scavenging activities was ethyl acetate fraction > crude ethanolic extract > n-butanol fraction > chloroform fraction > aqueous fraction. Similarly, ethyl acetate fraction of both fruits and leaves exhibited more antimicrobial activity as compared to that of ethanolic extract as revealed from agar well diffusion method with diameter of zone of inhibi¬tion of 14.0±1.41 mm, 21.0±1.41 mm, 14.0±1.41 mm, 14.5±0.71 mm in fruits and 18.0±1.41 mm, 22.5±2.12 mm, 15.5±2.12 mm, 14.5±3.53 mm in leaves against B. subtilis, S. aureus, E. coli, K. pneumoniae, respectively. MIC values for fruits were 3.125 mg/ml, 0.375 mg/ml, 3.125 mg/ml, 3.125 mg/ml and for leaves were 1.5625 mg/ml, 0.19 mg/ml, 0.78 mg/ ml, 0.78 mg/ml against B. subtilis, S. aureus, E. coli, K. pneumoniae, respectively. Conclusion: The present study provides the evidence for comparative antioxidant and antibacterial potential of ethanolic extracts of fruits and leaves of T. bellerica. . Moreover, leaves can be promoted to be used for therapeutics and natural antioxidants.

10aAntimicrobial10aAntioxidant10aEthanolic extract10aFruits10aLeaves10aTerminalia bellerica1 aChandel, Shikha, Rangra1 aKumar, Vikas1 aGuleria, Shikha1 aSharma, Nitin1 aSourirajan, Anuradha1 aKhosla, Prem, Kumar1 aBaumler, David, J.1 aDev, Kamal uhttps://www.phcogj.com/article/784