02358nas a2200253 4500008004100000245016400041210006900205260001800274300001400292490000700306520154100313653002501854653001301879653002201892653001001914653002101924100002301945700001601968700001901984700002102003700001702024700002402041856003902065 2018 eng d00aAntioxidant Activity, Total Phenolic and Flavonoid Content of Berberis aristata DC. and Berberis thomsoniana C.K. Schneid. from Sagarmatha National Park, Nepal0 aAntioxidant Activity Total Phenolic and Flavonoid Content of Ber cNovember 2018 as167-s1710 v103 a

Background: Medicinal plants are the major sources of the crude drugs and natural antioxidants. Berberis species have been used in the treatment of various ailments around the world including Nepal. However, systematic study on phytochemistry and pharmacology of Berberis species from Nepal is lacking. Objectives: To evaluate the antioxidant activity, total phenolic and flavonoid contents of Berberis aristata and Berberis thomsoniana from Sagarmatha National Park. Materials and Methods: Antioxidant activity was measured through 2, 2-Diphenyl-1-Picrylhydrazyl assay. Total phenolic and flavonoid content were estimated using Folin–Ciocalteau and aluminum chloride method respectively. Results: Samples showed dose-dependent radical scavenging activity. Radical scavenging activity of the methanolic extracts of different parts of B. aristata and B. thomsoniana ranged from 19.38 to 98.47%, with leaf extracts of B. thomsoniana showing the strongest activity. The total phenolic content of the samples varied from 11.04 to 65.30 mg GAE g-1 dry weight whereas total flavonoid content was in between 2.4 to 16.46 mg quercetin/g dry weight. Conclusion: Among the tested samples, leaf extracts of B. thomsoniana showed the strongest antioxidant activity and contained the highest amount of total phenolic and flavonoid content.

10aantioxidant activity10aBerberis10aFlavonoid content10aNepal10aPhenolic content1 aBhatt, Lok, Ranjan1 aWagle, Bina1 aAdhikari, Minu1 aBhusal, Santoshi1 aGiri, Anjana1 aBhattarai, Shandesh uhttps://www.phcogj.com/article/770