02412nas a2200217 4500008004100000245010300041210006900144260001600213300001400229490000700243520171700250653002501967653003301992653001602025653002702041653002802068100002102096700001602117700002202133856003902155 2018 eng d00aArginase Inhibitory and Antioxidant Activities of Caesalpinia coriaria (Jacq.) Willd. Bark Extract0 aArginase Inhibitory and Antioxidant Activities of Caesalpinia co cAugust 2018 a1174-11790 v103 a

Objective: The aim of this study was to investigate the arginase inhibitory and the antioxidant activities of the bark extract of Caesalpinia coriaria (Jacq.) Willd. (Dewi tree). Methods: The bark of Dewi tree was extracted successively under reflux condition with n-hexane, ethyl acetate, and methanol. Each extract was tested for its activity in inhibiting arginase activity by measuring the quantity of urea produced in the reaction mixture using a microplate reader. The active extracts were determined for their total flavonoid content followed by antioxidant activity by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method using ultraviolet-visible spectrophotometry with ascorbic acid as standard. Phytochemical screening was conducted to determine the presence of alkaloids, saponins, flavonoids, tannins, and steroids. Results: Arginase inhibitory activity test showed that the ethyl acetate and methanol extracts have average inhibition values of 14.43 and 33.59%, respectively, at concentration of 100 μg/mL. The total flavonoid content of the methanol and ethyl acetate extract were 7.75 and 6.30 mgQE/g sample, respectively. The methanol and ethyl acetate extracts showed antioxidant activity with an IC50 values of 4.720 and 3.647 μg/mL, respectively. The ethyl acetate extract contained flavonoid, tannin, saponin, and steroid, while the methanol extract contained flavonoid, tannin, and saponin. Conclusion: In conclusion, C. coriaria bark extracts possessed low arginase inhibitory activity. The methanol and ethyl acetate extracts have good antioxidant activity.

10aantioxidant activity10aArginase inhibitory activity10aC. coriaria10aPhytochemicalscreening10atotal flavonoid content1 aWulansari, Arini1 aElya, Berna1 aNoviani, Arikadia uhttps://www.phcogj.com/article/736