02769nas a2200229 4500008004100000245011000041210006900151260001600220300001400236490000700250520205500257653002702312653002502339653002202364653002102386653001302407653002602420100001902446700001802465700001702483856003902500 2018 eng d00aEndophytic Fungi Isolated from Crocus sativus L. (Saffron) as a Source of Bioactive Secondary Metabolites0 aEndophytic Fungi Isolated from Crocus sativus L Saffron as a Sou cAugust 2018 a1143-11480 v103 a

Introduction: Endophytic fungi are becoming an important source of new natural bioactive products. Many interesting endophytic fungi have been isolated from traditional medicinal plants. Crocus sativus L. (saffron) is one of the most expensive and rarest spices in the world, used as a dye, aroma and for medicinal purposes. This study reports on the molecular characterization of endophytic fungi isolated from roots of saffron plants growing in Taliouine- Morocco, and the examination of the antibacterial and antioxidant activities of secondary metabolites extracted from these endophytes. Methods and Material: The fungi were isolated from sterilized saffron root fragments and identified using internal transcribed spacer (ITS) rRNA gene sequence analysis. Three endophytes were fermented and extracted with ethyl acetate (EtOAc) and chloroform (CHCl3). The antibacterial activity of fungi extracts was evaluated using the Agar Diffusion Method against six strains, Bacillus sp., Stenotrophomonas sp., Pseudomonas putida, Pantoea sp., Luteibacter sp. and Escherichia coli. The antioxidant activity was tested by using the α,α-diphenyl-β-picrylhydrazyl (DPPH) analysis and β-Carotene bleaching test (BCBT) methods. Results: Sixty fungal isolates were recovered and purified from saffron roots. ITS rDNA sequences were 99-100% identical to three different species: Rhizopus oryzae, Aspergillus fumigatiaffinis and Aspergillus niger. Both Rhizopus oryzae extracts had high antibacterial activity against most of the bacteria tested, while Aspergillus niger and Rhizopus oryzae extracts showed an antioxidant capacity using DPPH and BCBT methods, respectively. Conclusion: The fungal endophytes inhabiting saffron roots could be a potential source of natural plant bioactive secondary metabolites especially for antibacterial and antioxidant purposes.

10aAntibacterial activity10aantioxidant activity10aCrocus sativus L.10aEndophytic fungi10aITS rDNA10aSecondary metabolites1 aChamkhi, Imane1 aSbabou, Laila1 aAurag, Jamal uhttps://www.phcogj.com/article/732