02429nas a2200217 4500008004100000245013600041210006900177260001600246300001400262490000700276520170300283653001601986653002002002653002502022653002002047653002902067100002802096700002202124700002602146856003902172 2018 eng d00aChemical Characteristics and In vitro Antidiabetic and Antioxidant Activities of Premna serratifolia L. Leaf Infusion and Decoction0 aChemical Characteristics and In vitro Antidiabetic and Antioxida cAugust 2018 a1114-11180 v103 a

Introduction: Leaves of Premna serratifolia L. (local name: arogo) is well known as food ingredient for fish/meat-based soup in Tentena, Indonesia. Evaluation of its bioactivities is needed. Objective: This study aimed to evaluate the α-glucosidase inhibitory and antioxidant activities of infusion and decoction of P. serratifolia leaves. Methods: The leaf samples were prepared by infusion and decoction and analysed for their α-glucosidase inhibitory and antioxidant activities, as well as total phenolic contents and LC-MS profiles. Results: The total phenolic content of the decoction was higher than the infusion. The UV spectra of both infusion and decoction showed peak at 325 nm and shoulder at 285 nm. Their LC-MS yielded one dominant component at 132 (100) m/z fragment, which was tentatively identified as caffeic acid. Both infusion and decoction extracts exhibited strong α-glucosidase inhibition with IC50 of 4.27 and 0.046 μg GAE/ml, respectively. The IC50 for DPPH scavenging activity of the infusion and decoction were similar; 6.82 and 7.28 μg GAE/ml, respectively. But the IC50 of the infusion and decoction for ferrous ion chelating activities were different; 161.66 and 28.34 μg GAE/ ml, respectively. The α-glucosidase inhibitory and antioxidant activities were related to the phenolic content. Conclusion: The study concludes that both infusion and decoction of arogo leaves have antidiabetic effect and potential antioxidant activity. The decoction of the leaves may increase its antidiabetic capacity.

10aAntioxidant10aAqueous extract10aInhibition mechanism10aP. serratifolia10aα-glucosidase inhibitor1 aTimotius, Kris, Herawan1 aSimamora, Adelina1 aSantoso, Adit, Widodo uhttps://www.phcogj.com/article/723