02500nas a2200241 4500008004100000245012700041210006900168260001600237300001200253490000700265520174200272653001702014653001702031653001702048653001602065653000902081653002402090100002802114700002502142700002102167700003102188856003902219 2018 eng d00aQuantitative and Optimization of Phenolic Acid Extracted from Pomegranate by High Performance Liquid Chromatography (HPLC)0 aQuantitative and Optimization of Phenolic Acid Extracted from Po cAugust 2018 a969-9720 v103 a

Objective: Pomegranate is scientifically known as Punica granatum L. which is a nutrient dense fruit rich in phytochemical compounds. Phenolic content is the main compound attribute for the most of the functional properties in pomegranate. The aim of this study is to quantify and optimize the composition of phenolic acids extracted from pomegranate extract by using High-performance Liquid Chromatography (HPLC). Method: The pomegranate extracted with three different methods by using two different solvents which is 50% ethanol and water. The methods were blended (aril+ seed), Soaking (aril+ seed) and soaking + squeezed manually. HPLC-PDA was used as equipment to quantify and optimize the phenolic acids extracted from pomegranate. Result: Validation method of HPLC was analysed according to the percentage of recovery, LOD, LOQ and coefficient correlation. Result showed that GA was detected in all sample from different method of extraction applied while CA compound not detected in any of extraction method applied. FA compound was only detected in blended method by 50% ethanol and water as solvent while EA compound was detected only in water extraction of all three methods applied. Conclusion: As a conclusion, according to the standard calibration data curve showed that this method proved to detect and quantify the targeted compounds. By comparing the data obtained from this study, it showed that water blended extract method is significantly higher content of targeted compound except for the CA compound. To the best of our knowledge, this sample can be a valuable source of antioxidant for better used in health benefits.

10aCaffeic acid10aEllagic acid10aFerulic acid10aGallic acid10aHPLC10aPomegranate extract1 aNoratiqah, Jumli, Mimie1 aNorhaslinda, Ridzwan1 aAmin, Baig, Atif1 aKhalili, Rohin, Mohd Adzim uhttps://www.phcogj.com/article/693