02632nas a2200205 4500008004100000245014500041210006900186260001400255300001200269490000700281520199000288653000902278653000902287653001402296653001302310653001602323100002702339700002502366856003502391 2018 eng d00aAntioxidant Activity, Total Phenolic Content and Total Flavonoid Content of Water and Methanol Extracts of Phyllanthus species from Malaysia0 aAntioxidant Activity Total Phenolic Content and Total Flavonoid  cJune 2018 a677-6810 v103 a

Aims: The effects of 2 types of solvents, water and methanol were investigated to determine the presence of antioxidant activity, total phenolic content (TPC) and total flavonoid content (TFC) from three Phyllanthus species namely, Phyllanthus urinaria, Phyllanthus niruri and Phyllanthus debilis. Materials and Methods: The antioxidant activities were measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,20- azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays. The chemical contents of the Phyllanthus sp. were presented as total phenolic content (TPC) and total flavonoid content (TFC). Statistical analysis used: All statistical analysis was conducted using SPSS for Windows, Version 22. All data were presented as mean ± standard deviation. Results: Our result showed that P. urinaria showed higher TPC, followed by P. debilis and P. niruri for both methanol and water extracts. Similarly, P. urinaria showed higher TFC than P. debilis and P. niruri. The antioxidant activity by using 1,1-diphenyl-2-picrydydrazyl (DPPH) assay showed EC50 of samples ranged from 15.8 to 29.3 μg/mL for methanol extract and 33.5 to 73.0 μg/mL for water extract. The 2,20- azinobis (3-ethylbenzothiazoline- 6-sulphonic acid) (ABTS) assay showed EC50 ranges which were from 11.2 to 26.0 μg/mL for methanol extract and 13.5 to 37.4 μg/mL for water extract. Conclusion: Methanol extract showed higher TPC, TFC value and lower EC50 values for antioxidant activities when compared to water extract. In both methanol and water extracts, P. urinaria had higher TPC and TFC value and lower EC50 for both DPPH and ABTS assay followed by P. debilis and P. niruri.

10aABTS10aDPPH10aFlavonoid10aphenolic10aPhyllanthus1 aZain, Siti, Nur Dalila1 aOmar, Wan, Adnan Wan uhttp://fulltxt.org/article/649