02054nas a2200217   4500008004100000245018400041210006900225260001300294300001200307490000700319520133900326653002301665653001701688653001001705653001401715653001501729100002001744700002201764700001501786856003501801       2018                        eng d00aHigh Performance Thin Layer Chromatographic Analysis for the Simultaneous Quantification of Two Polyphenolic Biomarkers in Methanolic Fraction of Bauhinia tomentosa L. Floral Buds0 aHigh Performance Thin Layer Chromatographic Analysis for the Sim  cMay 2018  a773-7770 v103 a<p style="text-align: justify;"><strong>Objectives:</strong> A high-performance thin layer chromatography (HPTLC) method for the simultaneous quantitative determination of caffeic acid and quercetin in methanolic fraction of <em>Bauhinia tomentosa</em> L. floral buds was developed for the first time. <strong>Method:</strong> For achieving good separation, a mobile phase of toluene: ethyl acetate: formic acid (7:3:0.5, v/v/v) was used. The densitometric determination was carried out at 366 nm in reflection/absorption mode. The calibration curves were linear in the range of 100-600 ng per spot for caffeic acid and quercetin. <strong>Results:</strong> During the analysis methanolic fraction of <em>Bauhinia tomentosa</em> L. floral buds showed the pres&not;ence of caffeic acid (0.02%) and quercetin (0.018%). <strong>Conclusion:</strong> The proposed method is simple, precise, specific, accurate, less time consuming and cost effective. The statistical analysis of data obtained proves that the method is reproducible and selective and can be used for routine analysis of reported phenolic compounds in crude drug and extracts. The simultaneous quan&not;tification of these compounds has not yet been reported in floral buds of <em>Bauhinia tomentosa</em> which may be utilized for the proper standardization of the plant.</p>10aBauhinia tomentosa10aCaffeic acid10aHPTLC10aQuercetin10aValidation1 aGupta, Abhishek1 aDwivedi, Harinath1 aRawat, AKS  uhttp://fulltxt.org/article/668