03589nas a2200241 4500008004100000245018600041210006900227260001400296300001200310490000700322520276000329653000903089653002703098653002803125653002203153653003403175653002303209653002503232653001203257100002203269700002103291856003503312 2018 eng d00aAn Interactive Antimicrobial Activity of Embelica officinalis Gaertn. Fruit Extracts and Conventional Antibiotics against Some Bacterial Triggers of Autoimmune Inflammatory Diseases0 aInteractive Antimicrobial Activity of Embelica officinalis Gaert cJune 2018 a654-6620 v103 a

Background: Embelica officinalis Gaertn. is an Indian plant which is known for its therapeutic properties. It is especially well known as a component of the Ayuverdic medicine Triphala. This study focuses on the growth inhibitory activity of E. officinalis fruit extracts against some bacterial triggers of autoimmune inflammatory diseases, both alone and in combination with conventional antibiotics. Methods: E. officinalis fruit powder was extracted with solvents of varying polarity and screened for bacterial growth inhibition by disc diffusion assay. The minimum inhibitory concentration (MIC) was quantified by both liquid dilution and disc diffusion techniques. To screen for combinatorial effects, the E. officinalis fruit extracts were combined with a range of conventional antibiotics and tested against each bacteria using a liquid dilution assay. Toxicity was examined using Artemia nauplii and HDF bioassays. Results: The ethyl acetate E. officinalis fruit extract displayed the strongest growth inhibitory activity against all of the bacterial triggers of autoimmune inflammatory disease. This extract was a particularly potent inhibitor of P. aeruginosa growth, with an MIC values as low as 264 μg/mL. The ethyl acetate extract was also a moderate to strong growth inhibitor of P. mirabilis, K. pneumonia and A. baylyi, with MIC values generally 1000-1500 μg/mL. The methanolic and aqueous extracts also inhibited the growth of all bacteria, although generally with only moderate to low activity. Whilst no synergistic interactions were detected in combinations containing the E. officinalis fruit extracts and conventional antibiotics, a number of combinations produced additive effects. These combinations are beneficial as they provide enhanced antibacterial efficacy compared to treatment with the antibiotic or extract components alone. No antagonistic interactions were detected. Therefore, use of the extracts in combination with conventional antibiotics would not compromise the antibiotics efficacy. All extracts were nontoxic in the Artemia nauplii and HDF toxicity assays, further indicating their potential for medicinal use. Conclusion: The E. officinalis fruit extracts were moderate inhibitors of the bacterial triggers of selected autoimmune inflammatory diseases. Furthermore, the extracts potentiated the activity of chloramphenicol and tetracycline against otherwise resistant bacterial strains. Isolation of the active compounds and the potentiating agents may be beneficial in antibiotic drug design.

10aAmla10aankylosing spondylitis10aCombinational therapies10aIndian gooseberry10aMulti-drug resistant bacteria10amultiple sclerosis10arheumatoid arthritis10aSynergy1 aHutchings, Adrian1 aCock, Ian, Edwin uhttp://fulltxt.org/article/646