02412nas a2200193 4500008004100000245013700041210006900178260001500247300001200262490000700274520171800281653003801999653002902037653002902066100003002095700002802125700003002153856003502183 2018 eng d00aStudies on Positive and Negative ionization mode of ESI-LC-MS/ MS for screening of Phytochemicals on Cassia auriculata (Aavaram Poo)0 aStudies on Positive and Negative ionization mode of ESILCMS MS f cMarch 2018 a457-4620 v103 a

Background: Cassia auriculata (Avaram) is an important medicinal plant in India Improved awareness in medicinal flowers has led to an increased need for efficient extraction methods and screening of flavonoid derivatives. Objective: To standardization of extraction solvent system and Characterization of flavonoids through positive and negative electrospray ionization mode using LC-MS/MS from Cassia auriculata extract. Materials and Methods: The different solvents like Methanol, Water, Acetonitrile, Ethyl Acetate, Ethanol, Chloroform, Hexane, Acetone Diethyl ether used frot he identification of flavonoids (Gallic acid, Theanine, Theobromine, Theophylline, Caffeic acid, Caffeine, Ferulic acid, Theacrine, Catechin, Quercetin, EpiGallo Catachin, catechin gallate, Epicatachin gallate and Quercetin hexoside. Results: Based on the peak area percentage the extraction solvent was standardized. The percentage of relative & absolute intensity of screened flavonoids was observed using LC-MS in positive and negative electrospray ionization. The results show that the methanol extract has more percentage of peak area, relative intensity and absolute intensity. The MS results showed that the negative ionoization has more intensity values of flavonoids and the signal-to-noise ratio was high in negative ionization mode compare to positive mode. Conclusion: Based on the results the methanol is the suitable extraction solvetnt and negative ionization mode of ESI-LC-MS/MS was appropriate for the screening of flavonoids on Cassia auriculata flower extracts.

10aCaucalis platycarpos L.; Methanol10aEelectrospray ionization10aFlavonoids; UHPLCESI- MS1 aRamakrishnan, Paranthaman1 aKalakandan, Sureshkumar1 aPakkirisamy, Muthukumaran uhttp://fulltxt.org/article/508