02717nas a2200229 4500008004100000245008600041210006900127260001700196300001200213490000700225520199300232653002902225653001502254653003402269653002302303653002102326100002802347700002902375700002302404700002502427856003502452 2017 eng d00aPharmacognostic Specification and Mangiferin Content of Aquilaria crassna Leaves.0 aPharmacognostic Specification and Mangiferin Content of Aquilari cJanuary-2018 a293-2980 v103 a

Background: Aquilaria crassna Pierre ex Lecomte (Thymelaeaceae) has been used as a medicinal plant in many aspects. Previous research has revealed that A. crassna leaves contain mangiferin as an active compound. Although the active component has been investigated, the pharmacognostic specification and quantification of mangiferin from A. crassna leaves have never been established. Objective: The current study aimed to conduct and develop a pharmacognostic standard according to WHO guidance as well as the validated method for quantifying mangiferin content. Materials and Methods: Dried A. crassna leaves from 15 separated locations throughout Thailand were investigated for pharmacognostic specification. Their mangiferin contents were quantitatively analysed by TLC densitometry with win CATS software. Results: Macroscopic-, microscopic- characteristics and TLC fingerprinting combined with physicochemical parameters were reported in this study. The loss on drying, moisture content, and total ash content as well as acid-insoluble ash content were determined to be 8.62 ± 0.13, 8.16 ± 0.14, 6.82 ± 0.09 and 1.49 ± 0.03%, respectively. Ethanol- and waterextractive values were found to be 9.05 ± 0.39 and 16.94 ± 0.22 %, respectively. In addition, the validation method for quantifying the mangiferin content was developed. The contents of mangiferin in A. crassna leaf extract determined by TLC-densitometry and TLC-image analysis were found to be 1.2992 ± 0.5980 and 1.3036 ± 0.5874 % by dried weight, respectively. The results between these two analytical methods were shown to have an insignificant difference. Conclusion: This study provides the necessary information for authentication and standardisation of A. crassna leaves.

10aAquilaria crassna leaves10aMangiferin10aPharmacognostic specification10aTLC image analysis10aTLC-densitometry1 aThitikornpong, Worathat1 aOngpipattanakul, Boonsri1 aPalanuvej, Chanida1 aRuangrungsi, Nijsiri uhttp://fulltxt.org/article/481