02439nas a2200229 4500008004100000245011900041210006900160260001700229300001200246490000700258520174400265653002802009653002002037653001402057653001502071653001702086653001302103100002002116700002002136700001802156856003502174 2018 eng d00aAndrographolide Induced Apoptosis in NALM-6 Cells Mediated Through the Cell Cycle Arrest and Nuclear Fragmentation0 aAndrographolide Induced Apoptosis in NALM6 Cells Mediated Throug cJanuary-2018 a210-2140 v103 a

Introduction: Andrographis paniculata is an herb widely cultivated in South and Southeastern Asia. It has been traditionally used to treat infections and other Physiological disorders for several hundreds. We investigated the anti-leukemic potential of Andrographolide (AGP) isolated from the leaves of this plant against an array of cancer cells to investigate its most efficacies in a particular cancer type. Methods: AGP was isolated from Andrographis paniculata leaves by using column chromatography. The structure was further determined by LC-MS, 1H NMR and 13C NMR. AGP was initially tested against four different cancer cell lines, namely NALM-6 (pre B-ALL), K562 (CML), A549 (lung carcinoma) and MCF-7 (breast carcinoma) using MTT assay at different time points and different concentrations. The effect of the isolated biomolecule was also investigated in inducing apoptosis through the study of cell cycle progression using flow cytometry by PI staining and nuclear fragmentation pattern by DAPI staining and fluorescence microscopy. Results: the spectral analysis of the isolated bio-molecule assured that the compound was AGP. MTT assay data indicated that AGP was most potent to induce cytotoxicity in NALM-6 cells. Further investigation revealed that it effectively induced apoptosis by arresting cell cycle progression and increased the nuclear break down in NALM- 6 leukemic cells. Conclusion: Our study efficiently demonstrated that the AGP isolated from Andrographis paniculata induced apoptosis in NALM-6 cells, which could be used in the therapeutic intervention of leukemia in the future.

10aAndrographis paniculata10aAndrographolide10aApoptosis10aCell cycle10aCytotoxicity10aLeukemia1 aSarkar, Swadesh1 aGopal, Priya, K1 aPaul, Santanu uhttp://fulltxt.org/article/466