02687nas a2200289 4500008004100000245008500041210006900126260001700195300001200212490000700224520188100231653001602112653000902128653000902137653001502146653001302161653001802174100003602192700001902228700001802247700003302265700001302298700001402311700002202325700001502347856003502362 2018 eng d00aAnthelmintic Activity of Fruit Extract and Fractions of Piper longum L. In vitro0 aAnthelmintic Activity of Fruit Extract and Fractions of Piper lo cJanuary 2018 a333-3400 v103 a

The present study was aimed to assess the in vitro ovicidal, larvicidal and adulticidal activity of methanolic extract and its fractions from fruits of Piper longum against strongyle ova, larvae and adult amphistomes respectively. The fruits of P. longum was identified and the accession number 006 was obtained. The phytochemical analysis revealed the presence of tannins, flavonoids, glycosides, phenolics, diterpenes and triterpenes in extract and fractions of P. longum. The extract and fractions were diluted serially in 6.25 per cent tween 80 to obtain concentrations of 500, 250, 125, 62.5, 31.25, 15.63, 7.81, 3.91 and 1.95 mg/mL. Ivermectin and thiabendazole at 10 μg/mL acted as positive controls and 6.25 per cent tween 80 as negative control. The methanolic extract was highly active against ova with IC50 of 0.026 mg/mL. The n-hexane fraction was potent in inducing larval mortality with IC50 of 1.383 mg/mL while chloroform fraction inhibited larval migration with IC50 of 1.796 mg/mL. Amphistomes were highly sensitive for methanolic extract of P. longum which possessed IC50 of 5.493 mg/mL Based on IC50 values, the methanolic extract was found to be most potent while chloroform fraction was effective against ova, larvae and also adults. GCMS analysis of potent methanolic extract revealed the presence of piperidinone, hydrocinnamic acid, ethylhexahydro azepine, methyleugenol, hexadecanoic acid and caryophyllene oxide which may have contributed for the anthelmintic activity. The acute oral toxicity study revealed mild vascular changes in liver. From the present study, it can be concluded that chloroform fraction of P. longum possessed maximum broad spectrum anthelmintic activity comparable to controls.

10aAdulticidal10aGCMS10aIC5010aLarvicidal10aOvicidal10aPiper longum.1 aKoorse, Krishnaprasad, Ganapati1 aSamraj, Sujith1 aJohn, Preethy1 aNarayanan, Priya, Manakkulap1 aSS, Devi1 aPTA, Usha1 aSunilkumar, Surya1 aVL, Gleeja uhttp://fulltxt.org/article/487