01967nas a2200241 4500008004100000245016000041210006900201260001800270300001200288490000700300520113300307653001501440653002601455653003101481653002201512100003001534700002601564700002401590700002301614700002901637700002401666856003501690 2018 eng d00aCell-free Supernatant from Exiguobacterium acetylicum Isolated from Water Cabbage (Pistia stratiotes) Roots Inhibits Bacillus subtilis and Escherichia coli0 aCellfree Supernatant from Exiguobacterium acetylicum Isolated fr cDecember 2017 a198-2010 v103 a

Introduction: The study was carried out to isolate and identify potential antibiotic-producing bacteria associated with water cabbage (Pistia stratiotes) roots collected from Pampanga River, Pampanga, Philippines. Seven (7) bacterial colonies were randomly chosen at the 10-6 dilution factor. Antibiotic sensitivity test using agar well method revealed that only one isolate out of 7 selected colonies can inhibit the growth of the test organisms. Specifically, the isolate (namely T4) supernatant inhibited E. coli and B. subtilis but not S. aureus. T4’s ability to inhibit E. coli was comparable with that of Tetracycline (positive control). Surprisingly, its inhibition of B. subtilis is significantly higher than that of Tetracycline. 16S rRNA gene sequence analysis using NCBI Basic Local Alignment Search Tool revealed 99% similarity of the isolate (T4) with Exiguobacterium acetylicum, a gram-positive, antibiotic-producing bacterium previously isolated from an apple orchard rhizosphere.

10aAntibiotic10aCell-free supernatant10aExiguobacterium acetylicum10aPistia stratiotes1 aRanganathan, Ashok, Kumar1 aPeƱa, Renato, A Dela1 aMalison, Marilyn, T1 aPunzalan, Oscar, V1 aPangilinan, Christian, R1 aGracilla, Daniel, E uhttp://fulltxt.org/article/420