02617nas a2200253   4500008004100000245016100041210006900202260001800271300001200289490000700301520181600308653001002124653002602134653001902160653001602179653001702195100001602212700001802228700001802246700001802264700002702282700001902309856003502328       2018                        eng d00aSimultaneous Quantification of Bioactive Triterpene acids (Ursolic acid and Oleanolic acid) in Different Extracts of Eucalyptus globulus (L) by HPTLC Method0 aSimultaneous Quantification of Bioactive Triterpene acids Ursoli  cDecember 2017  a179-1850 v103 a<p style="text-align: justify;"><strong>Objective:</strong> To develop a novel analytical method for simultaneous determination of two triterpenic acids by high-performance thin layer chromatography in methanol and dichloromethane extracts of <em>Eucalyptus globulus</em> leaf. Ursolic acid was also isolated from <em>Eucalyptus globulus</em> leaf. <strong>Materials and Methods:</strong> Two triterpenic acids (ursolic and oleanolic acid) were extracted using methanol and dichloromethane as the extraction solvents. Study for total triterpenoids present in <em>Eucalyptus globulus</em> leaves was carried out which shows considerable amount of terpenoids present. Because of the similarity of chemical structure, the prechromatographic derivatization was necessary to separate these triterpenic acids. The samples were treated by 1% iodine solution in chloroform directly on the chromatographic plate and developed with the mobile phase consisting of petroleum ether, ethyl acetate and acetone (7.8:2.2:0.1, v/v/v). After drying, the plates were sprayed with 10% (v/v) ethanol solution of sulfuric acid and heated to 120 &deg;C for 3 min. Quantification was performed in absorbance/transmittance mode at a wavelength of 345 nm. The developed HPTLC method was validated for linearity, precision and accuracy. <strong>Results:</strong> Correlation coefficient (<em>r<sup>2</sup></em> &gt; 0.99), R.S.D. values, detection limits as well as recovery values were found to be satisfactory. Ursolic acid was isolated from <em>E. globulus</em> leaves. The identification of isolated ursolic acid was done on the basis of Rf value (0.26) for HPTLC and peak interpretation for FT-IR. <strong>Conclusion:</strong> The method has been successfully applied in the analysis of both triterpenic acids in medicinal herbs.</p>10aHPTLC10aIodine derivatization10aOleanolic acid10aTriterpenes10aUrsolic acid1 aGupta, Arti1 aMaheta, Pooja1 aChauhan, Renu1 aPandey, Sonia1 aYadav, Jitendra, Singh1 aShah, Shailesh  uhttp://fulltxt.org/article/416