02765nas a2200301 4500008004100000245009100041210006900132260001200201300001200213490000600225520183600231653002302067653001502090653001402105653001302119653002002132653003002152100002702182700002802209700003002237700002402267700003202291700002302323700003102346700002002377700002802397856003802425 2015 eng d00aJackfruit (Artocarpus heterophyllus) seed extract exhibits fibrino(geno)lytic activity0 aJackfruit Artocarpus heterophyllus seed extract exhibits fibrino c01/2015 a171-1770 v73 a

Objective: The current study assesses the fibrinogen and fibrin clot hydrolyzing activities of aqueous seed extract of Jackfruit (AqSEJ). Methods: The protein banding pattern of AqSEJ (100 μg) was analyzed on SDS-PAGE. The proteolytic activity of AqSEJ was confirmed by spectrophotometer and zymography experiments. Fibrinogen, fibrin and plasma protein hydrolyzing activities of AqSEJ were analyzed on SDS-PAGE under reduced conditions. Plasminogen activation and indirect hemolytic activities was analyzed using spectrophotometer. The non-toxic property of AqSEJ was tested by edema, hemorrhage in experimental mice. Results: AqSEJ exhibited proteolytic activity and the specific activity was found to be 1.04 units/mg/min. Furthermore, AqSEJ non-specifically hydrolyzed Aα, followed by Bβ and γ chains of human fibrinogen and specifically hydrolyzed α polymer and α chain of partially cross linked human fibrin clot without affecting β chain and γ-γ dimer even up to the tested dose of 30 µg for the incubation period of 8 hours. Importantly, AqSEJ did not hydrolyze other plasma proteins and devoid of plasminogen activation property. The proteolytic activity of AqSEJ was completely neutralized by PMSF and IAA, while EDTA, EGTA, 1,10-Phenanthroline did not, suggesting the presence of serine and cysteine family proteases. Moreover, AqSEJ did not cause edema and hemorrhage in experimental mice up to the tested dose of 200 µg and non-toxic to RBC cells. Conclusion: AqSEJ hydrolyzes fibrinogen and fibrin clot and non-toxic in nature. Hence, this work showcases the potential applications of Jack fruit seed proteases in the treatment of thrombotic disorders.

10aFibrino(geno)lysis10aHemostasis10aJackfruit10aMoraceae10aNon-hemorrhagic10aSerine/cysteine protease.1 aGangaraju, Sowmyashree1 aManjappa, Bhagyalakshmi1 aSubbaiah, Girish, Kesturu1 aKempaiah, Kemparaju1 aShashidharamurthy, Rangaiah1 aPlow, Jane, Hoover1 aMartin, Sebastin, Santhosh1 aShinde, Manohar1 aSannaningaiah, Devaraja uhttps://www.phcogj.com/article/49