02340nas a2200241 4500008004100000245008700041210006900128260001800197300001200215490000700227520155900234653003001793653001601823653002801839653003101867100002201898700003201920700002801952700002601980700003102006700002602037856003502063 2018 eng d00aPhytochemical Screening, Antibacterial Activity, and Mode of Action on Morus nigra0 aPhytochemical Screening Antibacterial Activity and Mode of Actio cDecember 2017 a167-1710 v103 a

Introduction: The Morus nigra (Moraceae) known as black mulberry is a well-grown plant in Lembang, Indonesia. Previous studies showed that black mulberry fruit has activity as antibacterial and antioxidant. The aims of this study were to evaluate the antibacterial effect of ethanol extract from stem bark, fruit, and leaves of Morus nigra, MIC and morphological changes of the most active extract against Staphylococcus epidermidis and Propionibacterium acnes. Methods: Agar diffusion and microdilution assays were used to evaluate the antibacterial effect of the extract by measuring the zone of inhibition and the minimum inhibitory concentration (MIC) of the extract against the test bacteria. Scanning electron microscopy (SEM) was performed to evaluate the morphological changes induced by the extract in cellular membrane of the bacteria. Protein and ion leakage from the bacterial cells induced by the extract were also evaluated. Results: The antibacterial activity showed the most active extract was an ethanol extract of the stem bark against S. epidermidis and P. acnes with MIC value 4 mg/ml and 2 mg/ml, respectively. The extract also induced protein and ion leakage in test bacterial cells. SEM findings revealed that the extract induced potential cellular membrane damage in 4xMIC. Conclusion: The antibacterial activity was related to the damaged of bacterial cell wall.

10aCellular membrane damage.10aMorus nigra10aPropionibacterium Acnes10aStaphylococcus Epidermidis1 aAulifa, Diah, Lia1 aFitriansyah, Sani, Nurlaela1 aArdiansyah, Seno, Aulia1 aWibowo, Diki, Prayugo1 aJulata, Yolanda, Armiliani1 aChristy, Desty, Santi uhttp://fulltxt.org/article/414