02194nas a2200205   4500008004100000245009700041210006900138260001800207300001200225490000700237520152900244653002601773653002601799653004001825653003701865100002201902700000501924700002401929856003501953       2018                        eng d00aIn vitro Antibacterial Activity of Chitosan Nanoparticles against Mycobacterium tuberculosis0 aIn vitro Antibacterial Activity of Chitosan Nanoparticles agains  cDecember 2017  a162-1660 v103 a<p style="text-align: justify;"><strong>Background:</strong> Chitosan nanoparticles have gained growing interest for nanomedicine, biomedical engineering and development of new therapeutic with improved bioavailability, increased sensitivity and specificity, and reduced toxicity. <strong>Objective:</strong> The aim of the present study is to synthesis of the chitosan nanoparticles for antimycobacterial applications. <strong>Methods:</strong> Chitosan were isolated from the shrimp shell. Tripolyphosphate (TPP) will be used to prepare chitosan nanoparticles by ionotropic gelation method. The size and morphology of the chitosan nanoparticle was analyzed by scanning electron microscope (SEM). The broth microdilution method is used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of nanoparticle chitosan on strain <em>M. tuberculosis</em> H37Rv. <strong>Results:</strong> The SEM micrographs of the nano-magnetic chitosan showed that they were approximately uniform spheres and the rough surface morphology, have a solid dense cubical or rectangular structure. Using the broth microdilution susceptibility method, chitosan nanoparticle was found to have the antimycobacterial effects with a MIC value of 1200 &mu;g/mL whilst MBCs value of 2400 &mu;g/mL for <em>M. tuberculosis</em> H37Rv. Conclusion: The conclusion from the study was chitosan nanoparticle have potential as a source of lead compounds that may be developed further into antimycobacterial drugs.</p>10aChitosan nanoparticle10aM. tuberculosis H37Rv10aMinimal bactericidal concentration.10aMinimal inhibitory Concentration1 aWardani, Giftania1 a1 aSudjarwo, Sri, Agus  uhttp://fulltxt.org/article/413