02831nas a2200229 4500008004100000245010800041210006900149260001800218300001200236490000700248520208700255653002502342653001102367653003302378653001702411653002502428653002602453100003202479700002802511700002702539856003502566 2018 eng d00aEffects of Leucaena leucocephala (Lam.) de Wit Leaves Extracts in Culture of Human Umbilical Vein Cells0 aEffects of Leucaena leucocephala Lam de Wit Leaves Extracts in C cDecember 2017 a148-1530 v103 a

Oxidative stress can induce vascular endothelial dysfunction in diabetic and hyperlipidemia patients. Leucaena leucocephala (Lam.) de Wit has been reported of possessing antioxidant, antidiabetic and anticholiesterase activity; as well as the toxic substance called mimosine. Aims: To determine antioxidant effects of Leucaena leucocephala (Lam.) de Wit Leave extracts in oxidative stress induced vascular endothelial function. Methods and Material: Leave extracts were determined for antioxidant activity. Either or both of oxidized low density lipoprotein (oxLDL) and glucose were applied to induce oxidative stress condition in human umbilical vein cultured (HUVCs) to observe superoxide dismutase (SOD) activity, nitric oxide (NO) level and morphological changes. Results: Total polyphenol and flavonoid were 51.04 ± 0.91 mg GAE/g and 0.13 ± 0.01 mg catechin/g of dried weight (DW), respectively. Free radical reduction efficiency of crude extract observed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay showed IC50 value of 329.6 μg of vitamin C equivalent/mg of extracts. Frap value was showed 428.54 ± 15.32 mM FeII equivalent/g of DW. The result observing in HUVCs showed that comparing to the control, SOD activity, NO and MDA level were maintained in 0.05 mg/mL of L. leucocephala treated group, but NO and MDA level were lowered when comparing with oxLDL and glucose-induced oxidative stress. No change was observed in 0.05 mg/mL of L. leucocephala treated group, comparing with control group. Conclusion: This study has been performed to exhibit the antioxidant activity of L. leucocephala in endothelium functions and has been found to have an appropriate concentration at 0.05 mg/mL in reducing oxidative stress condition in impaired fasting blood glucose patients. Nevertheless, the optimal level for toxic activity in inhibition of cancer angiogenesis should be further investigated.

10aantioxidant activity10aDe Wit10aLeucaena leucocephala (Lam.)10aNitric oxide10aSuperoxide dismutase10aVascular endothelial.1 aChatchanayuenyong, Ratikorn1 aSujayanont, Patcharawan1 aVuttivirojana, Auranut uhttp://fulltxt.org/article/411