02206nas a2200217 4500008004100000245009400041210006900135260001800204300001000222490000700232520156900239653001401808653001101822653001801833653001501851653001201866100002401878700002601902700002501928856003501953 2018 eng d00aEvaluation of in vitro Antiviral Activity of Essential Oil Compounds Against Dengue Virus0 aEvaluation of in vitro Antiviral Activity of Essential Oil Compo cDecember 2017 a55-590 v103 a

Introduction: There are not effective drugs available for treatment of dengue fever despite intensive research on synthetic inhibitors. The search for active phytochemicals could serve for the discovery of new drugs. This study aims to evaluate the antiviral activity in vitro of compounds found in essential oils from medicinal plants. Materials and Methods: Nine synthetic-derived essential oil compounds were evaluated. Antiviral effect was screened measuring the reduction of viral NS1 and E proteins in HepG-2 and Vero cells. Results: β-Caryophyllene was identified as the most active compound, it reduced the virus serotype-2 replication in HepG-2 cells at IC50 of 22 ± 5.6 μM, and blocked replication of all four serotypes in Vero cells at IC50 between 8.0 μM and 15.0 μM. The selectivity indexes were between 5.3 and 10. According to results from time-in-addition assays, the antiviral effect of β-caryophyllene appears to be associated with interruption of early steps of the virus life cycle. Citral revealed modest antiviral effect, it reduced the virus serotype-2 (IC50 of 31 ± 4.5 μM) replication but not the other three serotypes. Seven terpenes did not reveal antiviral activity at maximum concentration of 30 μM. Conclusion: Research on compounds found in essential oils can contribute to the drug discovery effort for dengue. β-Caryophyllene could serve as a starting point.

10aAntiviral10aDengue10aEssential oil10aFlavivirus10aTerpene1 aFlechas, MarĂ­a, C.1 aOcazionez, Raquel, E.1 aStashenko, Elena, E. uhttp://fulltxt.org/article/366