03118nas a2200253 4500008004100000245015800041210006900199260001800268300001400286490000600300520229000306653001902596653001502615653001802630653001402648653001802662653001802680100002802698700003102726700002802757700002102785700002302806856003502829 2017 eng d00aAristolochia bracteolata Enhances Wound Healing in vitro through Anti-inflammatory and Proliferative Effect on Human Dermal Fibroblasts and Keratinocytes0 aAristolochia bracteolata Enhances Wound Healing in vitro through cNovember 2017 as129-s1360 v93 a

Objective: In the present study, we examined the effect of Aristolochia bracteolata extract on Human dermal fibroblast (HDF) and Human keratinocyte cell line (HaCaT) proliferation and migration during in vitro wound healing and its underlying mechanism. Method: A. bracteolata was collected and extracted using methanol. Cytotoxiciy effect of plant extract was determined by MTT assay in HDF and HaCaT. In vitro Scratch assay determined the effect of plant extracts on migration of cells and its underlying mechanism was determined by RT-PCR analysis. Result: The plant extract of A. bracteolata selectively inhibited proliferation of both the cells at higher concentration (>100 μg/mL) and at lower concentrations (<25 μg/mL), it exhibited linear and dose-dependent cell proliferation. IC50 value was 87.60±1.67 μg/mL for HDF and 85.50±1.65 μg/mL after 24 h treatment. In vitro scratch wound healing studies showed wound closure of 50.38%±1.39 and 69.81%±1.89 at a concentration of 25 μg/mL after 24 h and 48 h, respectively. The extract was tested for anti-inflammatory activity by determination of inhibitory activity on lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW 264.7 cell lines. We found that A. bracteolata has a strong inhibitory effect on the production of NO and tumor necrosis factor-α (TNF-α). The plant extract of A. bracteolata inhibited inducible nitric oxide synthase (iNOS) gene expression by lipopolysaccharide (LPS). To explore the mechanism responsible for the inhibition of iNOS, gene expression was analyzed by Real- Time PCR. A. bracteolata showed a decrease in the expression of pro-inflammatory cytokine mRNA in a concentration-dependent manner. Treatment with the plant extract resulted in enhanced expression of Collagen 1 a (I) and Collagen IV in HDFs by regulating the mRNA levels of extracellular matrix (ECM) proteins and Matrix metalloproteinase-2. Conclusion: Thus, the present investigation scientifically validates the use of A. bracteolata in wound healing.

10aA. bracteolata10aFibroblast10aKeratinocytes10aRAW 264.710aScratch assay10aWound Healing1 aGirija, Dinesh, Murugan1 aKalachaveedu, Mangathayaru1 aSubbarayan, Rajasekaran1 aJenifer, Preethi1 aRao, Suresh, Ranga uhttp://fulltxt.org/article/394