02342nas a2200193 4500008004100000245009400041210006900135260001200204300001200216490000600228520176600234653001602000653001902016653001402035653002302049100002102072700001702093856003802110 2015 eng d00aPogostemon cablin (Blanco) Benth. (Lamiaceae): It’s Ethnobotany & in vitro regeneration0 aPogostemon cablin Blanco Benth Lamiaceae It s Ethnobotany in vit c01/2015 a152-1560 v73 a

Objectives: Since the beginning of human civilization various herbal medicines are employed for healing human and animal. Pogostemon cablin (Blanco) Benth., locally known as Patchouli (Assamese) is a very important medicinal plants belongs to mint family i.e. Lamiaceae. The main aim of this study was to collect ethnobotanical information’s and to study its in vitro regeneration results. All possible ethnobotanical literatures have been cited here. Methods: In vitro propagation was achieved from leaf and nodal explants of Pogostemon cablin on MS medium. Results: Callus development and in vitro axillary shoot formation was successfully made in MS basal medium containing BA (4.0 mg/L), NAA (2.0 mg/L)+ IAA (1.0 mg/L) and BA (3 mg/L)+IAA (1 mg/L). MS basal medium containing IBA (0.1/L) and Kn (2.5 mg/L) was best for induction of multiple shoots within 4 weeks of culture. Combination of NAA (0.1 mg/L). Kn (0.1 mg/L) and CH (100 mg/L) was best for callus induction which later on formed multiple shoots and caused elongation of roots. Micro shoots of varied length were produced on MS medium. Rooted plantlets were successfully acclimatized in green house for 1 month and then were transferred to the field. Conclusion: It can be concluded that pogostemon cablin has immense ethno botanical importance. For its rapid multiplication, in vitro technique was found very successful. In MS medium supplemented with Kn 2.5 mg/L and IBA 0.1mg/L found maximum multiplication rate. In this proportion rates of shoot generation, leaf, rooting, callus formation was maximum.

10aEthnobotany10ain vitro study10aMS medium10aPogostemon cablin.1 aSharma, Pratibha1 aSarma, Jintu uhttps://www.phcogj.com/article/44