02997nas a2200289 4500008004100000245009800041210006900139260001900208300001200227490000600239520210500245653002202350653001802372653001602390653002302406653001602429100003302445700002302478700002802501700002502529700002602554700002902580700002602609700001802635700001902653856003502672 2017 eng d00aPhenolic Constituents, Anti-Inflammatory and Antidiabetic Activities of Cyperus laevigatus L.0 aPhenolic Constituents AntiInflammatory and Antidiabetic Activiti cSeptember 2017 a828-8330 v93 a

Background: Cyperus species are well known traditional plants and used for several diseases around the world. Aim of the Study: Our study aimed to identification of the phenolic constituents in addition to evaluation of different extracts of Cyperus laevigatus L as antioxidant, antiinflammatory and antidiabetic agents. Materials and Methods: The phenolic constituents were identified using spectroscopic techniques. The antioxidant activity was evaluated using in vitro DPPH assay. Total extract, methanol and EtOAc fractions were evaluated for their antiinflammatory activity using RAW 264.7 macrophages assay. Antidiabetic activity of the total extract was examined biochemically and histopathologically using streptozotocin-induced diabetic rats. Results: A new flavone, chrysoeriol 7-O-β-(6‴-O-acetyl-β-D-glucopyranosyl)-(1→4) glucopyranoside (1), along with seven knowns (2-8) were isolated from Cyperus laevigatus L. The structures of isolated compounds were established depending upon 1D, 2D-NMR and HR-ESI-MS. The MeOH and EtOAc fractions exhibited significant antioxidant activity while the isolated flavonoids exhibited from moderate to weak antioxidant activity. The total extract, MeOH and EtOAc fractions exhibited significant anti-inflammatory activity using LPS-stimulated RAW 264.7 macrophages model by decreasing of NO accumulation by 76 – 66% and 84 – 67%, of the original accumulation values with increasing concentrations in comparison with the reference drug, dexamethasone. The total extract exhibited antidiabetic activity in streptozotocin-induced diabetic rats and this effect was manifested by decreasing serum levels of glucose, glucagon and NO. It also increased level of insulin and promoted paraoxonase activity. Conclusion: These results proved that this plant may be multiple sources for medicinal natural drugs especially for anti-inflammatory and antidiabetic.

10aAnti-inflammatory10aAntidiabetic.10aAntioxidant10aCyperus Laevigatus10aNew Flavone1 aElshamy, Abdelsamed, Ibrahim1 aEl-Shazly, Mohamed1 aYassine, Yomna, Mahmoud1 aEl-Bana, Mona, Anwar1 aFarrag, Abdel-, Razik1 aNassar, Mahmoud, Ibrahim1 aSingab, Abdel, Nasser1 aNoji, Masaaki1 aUmeyama, Akemi uhttp://fulltxt.org/article/182