02288nas a2200253 4500008004100000245015300041210006900194260001900263300001200282490000600294520148600300653001701786653001701803653001601820653001501836653002101851653002701872100001801899700000501917700003401922700002201956700002101978856003501999 2017 eng d00aPhytochemical Screening and Content Determination of Different Species of Genus Caesalpinia belonging to Different Origin with Antidiabetic Activity0 aPhytochemical Screening and Content Determination of Different S cSeptember 2017 a743-7490 v93 a

Aim: The main aim of the study is to investigate the phytochemical screening of C. decapetala along with the content determination of different species of genus Caesalpinia with respect to their antidiabetic activity and identification of most bioactive species belonging to different origins. Methods: To achieve our goal different species of genus Caesalpinia collected from China and Pakistan were subjected to open column chromatography, High Pressure Liquid Chromatography (HPLC), antioxidant, and antidiabetic assays for evaluation. Results: From η-BuOH fraction of C. decapetala extract, eight compounds were isolated using open column chromatography and identified as apigenin-7-rhamnoside (1), 4-O-methylepisappanol (2), caesalpinol (3), daucosterol (4), astragalin (5), kaempferol (6), quercitrin (7), and naringin (8) using Nuclear Magnetic Resonance (NMR) spectroscopy. HPLC analysis of different species of genus Caesalpinia showed that the most active antidiabetic compound ‘quercitrin’ was present more in C. pulcherrima followed by decreasing order in C. sappan, C.decapetala, and C. bonduc. Conclusion: The results indicated that quercitrin is the most bioactive content and C. pulcherrima is most bioactive specie of China origin from genus Caesalpinia.

10aAntidiabetic10aAntioxidants10aCaesalpinia10aFlavonoids10aHPLC Fingerprint10aPhytochemical content.1 aParveen, Amna1 a1 aFarooqi, Muhammad, Qudratulla1 aKyunn, Whang, Wan1 aArshad, Muhammad uhttp://fulltxt.org/article/170